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Human Fibrinogen ELISA Kit (ab108842)

Submit a review Q&A (3)References (10)
Sandwich ELISA - ab108842 Fibrinogen Human ELISA Kit
  • Typical Standard Curve

Key features and details

  • Sensitivity: 0.35 µg/ml
  • Range: 0.494 µg/ml - 20 µg/ml
  • Sample type: Plasma
  • Detection method: Colorimetric
  • Assay type: Competitive
  • Reacts with: Human

製品の概要

  • 製品名

    Human Fibrinogen ELISA Kit
    Fibrinogen キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Overall 5.9%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Overall 10.4%

  • サンプルの種類

    Plasma

  • アッセイタイプ

    Competitive

  • 検出感度

    = 0.35 µg/ml

  • 検出範囲

    0.494 µg/ml - 20 µg/ml

  • 添加回収試験

    98 %

  • 全工程の試験時間

    3h 00m

  • ステップ

    Multiple steps standard assay

  • 種交差性

    交差種: Human

  • 製品の概要

    Human Fibrinogen in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibrinogen levels in plasma.


    A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Fibrinogen is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Fibrinogen captured in plate.


    Get better reproducibility in only 90 minutes with Human Fibrinogen ELISA Kit (ab208036) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • アプリケーション

    適用あり: Competitive ELISAmore details

  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 1 x 30ml
    2X Biotinylated Human Fibrinogen (Lyophilized) 1 vial
    Chromogen Substrate 1 x 7ml
    Fibrinogen Microplate (12 x 8 well strips) 1 unit
    Fibrinogen Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 11ml

  • 研究分野

  • 機能

    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.

  • 組織特異性

    Plasma.

  • 関連疾患

    Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
    Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.

  • 配列類似性

    Contains 1 fibrinogen C-terminal domain.

  • ドメイン

    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.

  • 翻訳後修飾

    The alpha chain is not glycosylated.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Phosphorylation sites are present in the extracellular medium.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 別名

    • FGA
    • FGB
    • FGG
    • Fib2
    • FIBA_HUMAN
    • Fibrinogen A alpha polypeptide
    • Fibrinogen alpha chain
    • Fibrinogen B alpha polypeptide
    • Fibrinogen beta chain
    • Fibrinogen G alpha polypeptide
    • Fibrinogen gamma chain
    • fibrinogen, B beta polypeptide
    • fibrinogen, G gamma polypeptide
    • fibrinogen, gamma polypeptide
    • Fibrinogen--alpha -polypeptide chain
    • Fibrinogen--beta -polypeptide chain
    • Fibrinogen--gamma-polypeptide chain
    see all

  • 参照データベース

    see all

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108842の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Competitive ELISA
Use at an assay dependent concentration.
特記事項
Competitive ELISA
Use at an assay dependent concentration.

画像

  • Sandwich ELISA - ab108842 Fibrinogen Human ELISA Kit
    Sandwich ELISA - ab108842 Fibrinogen Human ELISA Kit

    Fibrinogen measured in various samples showing quantity (micrograms) per mL of tested sample

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab108842.

参考文献 (10)

ab108842 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108842 は 10 報の論文で使用されています。

  • Kietsiriroje N  et al. Dietary fat intake is associated with insulin resistance and an adverse vascular profile in patients with T1D: a pooled analysis. Eur J Nutr 62:1231-1238 (2023). PubMed: 36495341
  • Sadat A  et al. Immunological and Oxidative Biomarkers in Bovine Serum from Healthy, Clinical, and Sub-Clinical Mastitis Caused by Escherichia coli and Staphylococcus aureus Infection. Animals (Basel) 13:N/A (2023). PubMed: 36899749
  • Kietsiriroje N  et al. Glucose variability is associated with an adverse vascular profile but only in the presence of insulin resistance in individuals with type 1 diabetes: An observational study. Diab Vasc Dis Res 19:14791641221103217 (2022). PubMed: 35657731
  • Pulcini S  et al. Apheresis Platelet Rich-Plasma for Regenerative Medicine: An In Vitro Study on Osteogenic Potential. Int J Mol Sci 22:N/A (2021). PubMed: 34445472
  • O'Mahoney LL  et al. Estimated glucose disposal rate as a candidate biomarker for thrombotic biomarkers in T1D: a pooled analysis. J Endocrinol Invest 44:2417-2426 (2021). PubMed: 33730349
  • Liang Y  et al. Coagulation cascade and complement system in systemic lupus erythematosus. Oncotarget 9:14862-14881 (2018). PubMed: 29599912
  • Campbell MD  et al. An additional bolus of rapid-acting insulin to normalise postprandial cardiovascular risk factors following a high-carbohydrate high-fat meal in patients with type 1 diabetes: A randomised controlled trial. Diab Vasc Dis Res 14:336-344 (2017). PubMed: 28322071
  • Egorov AI  et al. Vegetated land cover near residence is associated with reduced allostatic load and improved biomarkers of neuroendocrine, metabolic and immune functions. Environ Res 158:508-521 (2017). PubMed: 28709033
  • Al-Daghri NM  et al. Sex-specific vitamin D effects on blood coagulation among overweight adults. Eur J Clin Invest 46:1031-1040 (2016). PubMed: 27727459
  • Nguyen TT  et al. A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen. Int J Nanomedicine 10 Spec Iss:155-63 (2015). PubMed: 26347331

レビューと Q&A

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レビューを送る 質問を送る

1-3 of 3 Abreviews or Q&A

Question

For ab108841: can plasma/serum samples be used? with EDTA as anti-coagulant?
For ab171578, ab108842: can plasma/serum with EDTA as anti-coagulant be used with this kit?

Read More
Answer

Plasma treated with EDTA anticoagulant can be used with all three of these ELISA kits. However, if choosing between ab108842 and ab108841, the competitive ELISA ab108842 is preferable to ab108841 for this sample type. The SimpleStep ELISA ab171578 is preferable to both for plasma samples, given the relative simplicity of the protocol and the data for plasma samples that is presented on the datasheet.

Read More

Question

So, this means my data is usable??

Read More
Answer

Thank you for your reply.

Yes! Competitive ELISAs are a little different. Here is the background.

Competitive ELISA: Basic Principles

The central event of competitive ELISA is a competitive binding process executed by original antigen (sample antigen) and add-in antigen. The procedures of competitive ELISA are different in some respects compared with Indirect ELISA, Sandwich ELISA and Direct ELISA. A simplized procedure list is as follow:

Primary antibody (unlabeled) is incubated with sample antigen.
Antibody-antigen complexes are then added to 96-well plates which are pre-coated with the same antigen.
Unbound antibody is removed by washing the plate. (The more antigen in the sample, the less antibody will be able to bind to the antigen in the well, hence "competition.")
The secondary antibody that is specific to the primary antibody and conjugated with an enzyme is added.
A substrate is added, and remaining enzymes elicit a chromogenic or fluorescent signal.



For competitive ELISA, the higher the sample antigen concentration, the weaker the eventual signal. The major advantage of a competitive ELISA is the ability to use crude or impure samples and still selectively bind any antigen that may be present.

I hope this helps!

Read More

Question

Dear,

For My experiment, I need an ELISA for fibrinogen detection for mice and human for my some experiments. I just check on website that you have both type of ELISAs but i was not able to find the cross reactivity with opposite one. We will prefer an ELISA which may detect both human and mice fibrinogen (cross-reactive). Kindly provide me the information ASAP if available.

Read More
Answer

Thank you for you for your enquiry and your interest in our products.

I have conducted a search for you using our advanced search engine. Currently, we only have ELISA kits which are specific for either human (ab108841, ab108842) or mouse fibrinogen (ab108843, ab10844). Unfortunately, we do not have any kits which would recognize both species.

Apologies!

If you need any further assistance in the future, please do not hesitate to contact me.

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