Human FGF-21 ELISA Kit (ab125966)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 0.063 ng/ml - 0.5 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human FGF-21 ELISA Kit
FGF21 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 5.2% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 9.7% -
サンプルの種類
Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
20 pg/ml -
検出範囲
0.063 ng/ml - 0.5 ng/ml -
添加回収試験
101 %
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全工程の試験時間
5h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s FGF-21 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human FGF-21 in plasma, serum, and cell culture supernatants.
A FGF-21 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a FGF-21 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of FGF-21 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human FGF-21 Antibody 1 x 120µl Chromogen Substrate 1 x 7ml FGF-21 Microplate (12 x 8 well strips) 1 unit FGF-21 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. -
配列類似性
Belongs to the heparin-binding growth factors family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- FGF 21
- FGF-21
- Fgf21
see all -
参照データベース
- Entrez Gene: 26291 Human
- Omim: 609436 Human
- SwissProt: Q9NSA1 Human
- Unigene: 283015 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab125966の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Sandwich ELISA - FGF-21 Human ELISA Kit (ab125966)FGF-21 measured in biological fluids, background signal subtracted (duplicates +/- SD).
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Typical Standard CurveRepresentative Standard Curve using ab125966
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab125966 は 8 報の論文で使用されています。
- Cai M et al. Fibroblast Growth Factor 21 Relieves Lipopolysaccharide-Induced Acute Lung Injury by Suppressing JAK2/STAT3 Signaling Pathway. Inflammation 47:209-226 (2024). PubMed: 37864659
- Gijbels A et al. Plasma FGF21 Levels Are Not Associated with Weight Loss or Improvements in Metabolic Health Markers upon 12 Weeks of Energy Restriction: Secondary Analysis of an RCT. Nutrients 14:N/A (2022). PubMed: 36501091
- Jiang L et al. Serum fibroblast growth factor 21 level is increased in pre-eclampsia patients: Association with blood pressure and lipid profile. J Obstet Gynaecol Res 47:375-381 (2021). PubMed: 33073442
- Siemienowicz KJ et al. Pubertal FGF21 deficit is central in the metabolic pathophysiology of an ovine model of polycystic ovary syndrome. Mol Cell Endocrinol 525:111196 (2021). PubMed: 33556473
- Peláez-Jaramillo MJ et al. Impact of a Formulation Containing Unusual Polyunsaturated Fatty Acids, Trace Elements, Polyphenols and Plant Sterols on Insulin Resistance and Associated Disturbances. Diabetes Ther 11:229-245 (2020). PubMed: 31691133
- Mölzer C et al. Features of an altered AMPK metabolic pathway in Gilbert's Syndrome, and its role in metabolic health. Sci Rep 6:30051 (2016). Sandwich ELISA ; Human . PubMed: 27444220
- Dekker Nitert M et al. Placental fibroblast growth factor 21 is not altered in late-onset preeclampsia. Reprod Biol Endocrinol 13:14 (2015). Sandwich ELISA ; Human . PubMed: 25890271
- Dekker Nitert M et al. Increased placental expression of fibroblast growth factor 21 in gestational diabetes mellitus. J Clin Endocrinol Metab 99:E591-8 (2014). PubMed: 24432989