Human Ferritin ELISA Kit (ab108698)
Key features and details
- Sensitivity: 0.53 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Ferritin ELISA Kit
Ferritin Light Chain キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Control 48 < 5.4% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Control 48 < 6.1% -
サンプルの種類
Serum, Hep Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
0.53 ng/ml -
添加回収試験
98.66 %
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ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Human Ferritin ELISA kit is a competitive ELISA designed for the accurate quantitative measurement of Ferritin (FTL) in serum and plasma.
A 96-well plate has been precoated with anti-Ferritin IgG antibodies. Samples, standards and the Ferritin-HRP conjugate are added to the wells, where Ferritin in the sample and standards binds to the precoated antibody and added Ferritin-HRP conjugate binds to this antibody-AFP complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Ferritin in the sample and the intensity is measured at 450 nm.
Get results in 90 minutes with Human Ferritin ELISA Kit (FLT) (ab200018) from our SimpleStep ELISA® range.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 10X Washing Solution 1 x 50ml Anti-Ferritin IgG Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Anti-Ferritin-HRP conjugate 1 x 12ml Cover foil 1 unit Ferritin Control 1 x 1ml Ferritin Standard 0 - 0 ng/mL 1 x 3ml Ferritin Standard 1 - 5 ng/mL 1 x 1ml Ferritin Standard 2 - 20 ng/mL 1 x 1ml Ferritin Standard 3 - 100 ng/mL 1 x 1ml Ferritin Standard 4 - 400 ng/mL 1 x 1ml Ferritin Standard 5 - 800 ng/mL 1 x 1ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
研究分野
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機能
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. -
関連疾患
Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels. -
配列類似性
Belongs to the ferritin family.
Contains 1 ferritin-like diiron domain. - Information by UniProt
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別名
- Ferritin L chain
- Ferritin L subunit
- Ferritin light chain
see all -
参照データベース
- Entrez Gene: 2512 Human
- Omim: 134790 Human
- SwissProt: P02792 Human
- Unigene: 433670 Human
- Unigene: 713706 Human
関連製品
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アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108698の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Typical Standard Curve
Representative Standard Curve using ab108698
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab108698 は 9 報の論文で使用されています。
- Loria F et al. The effects of iron injection on blood doping biomarkers in dried blood spots. Drug Test Anal 15:444-448 (2023). PubMed: 36354188
- Sadat A et al. Immunological and Oxidative Biomarkers in Bovine Serum from Healthy, Clinical, and Sub-Clinical Mastitis Caused by Escherichia coli and Staphylococcus aureus Infection. Animals (Basel) 13:N/A (2023). PubMed: 36899749
- Raha-Chowdhury R et al. Impaired Iron Homeostasis and Haematopoiesis Impacts Inflammation in the Ageing Process in Down Syndrome Dementia. J Clin Med 10:N/A (2021). PubMed: 34209847
- Raha AA et al. Hepcidin Increases Cytokines in Alzheimer's Disease and Down's Syndrome Dementia: Implication of Impaired Iron Homeostasis in Neuroinflammation. Front Aging Neurosci 13:653591 (2021). PubMed: 33994996
- Richardson KJ et al. Mechanical sensitivity of red blood cells improves in individuals with hemochromatosis following venesection therapy. Transfusion 60:3001-3009 (2020). PubMed: 32939772
- Richardson KJ et al. Hemochromatosis alters the sensitivity of red blood cells to mechanical stress. Transfusion 60:2982-2990 (2020). PubMed: 32945551
- Ajepe AA et al. Prevalence and foetomaternal effects of iron deficiency anaemia among pregnant women in Lagos, Nigeria. PLoS One 15:e0227965 (2020). PubMed: 31971986
- Devos D et al. A ferroptosis-based panel of prognostic biomarkers for Amyotrophic Lateral Sclerosis. Sci Rep 9:2918 (2019). PubMed: 30814647
- Smith EM et al. High-dose vitamin D3 reduces circulating hepcidin concentrations: A pilot, randomized, double-blind, placebo-controlled trial in healthy adults. Clin Nutr 36:980-985 (2017). PubMed: 27402475