Human Fas Ligand ELISA Set (without plates) (ab83703)
製品の概要
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製品名
Human Fas Ligand ELISA Set (without plates) -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 44 pg/ml -
検出範囲
62.5 pg/ml - 2000 pg/ml -
全工程の試験時間
3h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Fas Ligand (Fas-L) Human in vitro ELISA Set is designed for the quantitative measurement of Fas-L in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The Fas L ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This ELISA Set is intended to measure Fas L in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
SPECIFICITY: Recognizes both natural and recombinant soluble human Fas-Ligand RANGE: 62.5pg/ml - 2000pg/ml SENSITIVITY: <12pg/ml
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Reagents
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 5 x 96 tests 10 x 96 tests Detection biotinylated anti-Fas Ligand antibody 1 x 0.55ml 2 x 0.55ml Fas Ligand Capture Antibody 1 x 0.5ml 2 x 0.5ml FAS Ligand Standard (Lyophilized) Yellow 5 vials 10 vials Ready-to-use TMB 2 vials 4 vials Streptavidin-HRP 1 vial 2 vials -
研究分野
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関連性
Fas / APO1 (CD95) is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumors. Tumor cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system. The full length of FasL contains 281 amino acids, consisting of the transmembrane domain, extracellular domain, and the cytoplasmic region. A metalloproteinase cleaves the membrane-bound FasL to produce soluble Fas (sFasL), which exists as a homotrimer. The sFasL shows lower cytotoxicity than membrane bound FasL. -
細胞内局在
Cell membrane: single pass type II membrane protein. Secreted protein: may be released into the extracellular fluid, probably by cleavage form the cell surface. -
別名
- Apoptosis (APO 1) antigen ligand 1
- APT1LG1
- APTL
see all -
参照データベース
- Entrez Gene: 356 Human
- Omim: 134638 Human
- SwissProt: P48023 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab83703の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Fas Ligand Human ELISA Set (ab83703)Fas Ligand standard curve ranging from 62.5 to 2000 pg/mL
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab83703 は論文での使用が確認できていません。