Human FAS Ligand ELISA Kit (CD95L) (ab45892)
Key features and details
- Sensitivity: 12 pg/ml
- Range: 62.5 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human FAS Ligand ELISA Kit (CD95L)
Fas Ligand キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Sample A 40 588.4pg/ml 22.6 3.8% Sample B 35 302.2pg/ml 14.6 4.5% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 12 pg/ml -
検出範囲
62.5 pg/ml - 2000 pg/ml -
全工程の試験時間
3h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Human FAS Ligand in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of FAS Ligand in Human serum, plasma, buffered solutions and cell culture media.
A monoclonal antibody specific for FAS Ligand has been coated onto the wells of the microtiter strips provided. Samples, including standards of known FAS Ligand concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for FAS Ligand are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of FAS Ligand present in the samples.
This kit will recognize both endogenous and recombinant Human FAS Ligand.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Biotinylated anti-FAS Ligand Red 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml Control Silver 2 vials 4 vials FAS Ligand Microplate (12 x 8 well strips) 1 unit 2 units FAS Ligand Standard (Lyophilized) Yellow 2 vials 4 vials HRP Diluent Red 1 x 12ml 1 x 23ml Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究分野
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関連性
Fas / APO1 (CD95) is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane-mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumours. Tumour cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system. -
細胞内局在
Cell Membrane (single-pass type II membrane protein) and Secreted -
別名
- Apoptosis (APO 1) antigen ligand 1
- Apoptosis antigen ligand
- APT1LG1
see all -
参照データベース
- Entrez Gene: 356 Human
- Omim: 134638 Human
- SwissProt: P48023 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab45892の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Typical Standard Curve
Representative standard curve using ab45892.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab45892 は 1 報の論文で使用されています。
- Qiu M et al. Transforming growth factor ß1 and Fas ligand synergistically enhance immune tolerance in dendritic cells in liver transplantation. J Surg Res 218:180-193 (2017). PubMed: 28985848