AB192144

Human Factor XII ELISA Kit

Recombinant
SimpleStep
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Human Factor XII ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Factor XII with a sensitivity of 0.1 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

Coagulation factor XII, Hageman factor, HAF, F12

7 Images

ELISA - Human Factor XII ELISA Kit (AB192144)

ELISA

Supplier Data

ELISA - Human Factor XII ELISA Kit (AB192144)

sELISA

Lab

Sandwich ELISA - Human Factor XII ELISA Kit (AB192144)

Interpolated concentrations of human Factor XII in serum in 96-well vs. 384-well plates

Interpolated concentration of native Factor XII was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 6,600 serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human Factor XII ELISA Kit (AB192144)

Example of human Factor XII standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Factor XII ELISA Kit (AB192144)

Serum from eight individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 30.4 μg/mL with a range of 21.8 – 40.3 μg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Factor XII ELISA Kit (AB192144)

Interpolated concentrations of native Factor XII in human urine samples.

The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 60.7 ng/mL in urine 12.5%.

sELISA

Supplier Data

Sandwich ELISA - Human Factor XII ELISA Kit (AB192144)

Interpolated concentrations of native Factor XII in human serum, plasma and cell culture supernatant samples.

The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 6,600, plasma (EDTA) 1 : 1,600, plasma (citrate) 1 : 1,600 and plasma (heparin) 1 : 8,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 25.0 μg/mL in serum, 21.7 μg/mL in plasma (EDTA), 21.0 μg/mL in plasma (citrate), and 32.1 μg/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human Factor XII ELISA Kit (AB192144)

Example of human Factor XII standard curve in Sample Diluent NS.

The Factor XII standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 0.1 ng/mL

検出範囲

0.23 - 15 ng/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human Factor XII ELISA Kit ab192144 is a rapid single-wash 90-min Sandwich ELISA to measure Human Factor XII in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, urine. This SimpleStep sensitivity is 0.1 ng/mL.

How the assay works

Human Factor XII SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Factor XII ELISA Kit ab192144 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Human Factor XII ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Factor XII antibody [EPR22292-151] - BSA and Azide free (Capture) ab259448
- Anti-Factor XII antibody [EPR22292-25] - BSA and Azide free (Detector) ab259448

Factor XII is a 80 kDa plasma serine protease involved in the coagulation cascade. Factor XII is activated by proteolysis and the product, Factor XIIa, goes on to activate Factor XI and prekallikrein in in vitro models of blood cogulation. Its involvement in the beginning of the coagulation cascade is only seen in in vitro studies, and is thought to have no effect on coagulation initiation in vivo. While Factor XII deficiency in humans is asymptomatic, Factor XII plays a role in later stages of clot formation. Deficiency of Factor XII affects the in vitro measurements of partial thromboplastin time in human blood, and its absence decreases the susceptibility of mice to thrombosis.

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精度

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" } ]

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回収率

[ { "sample": "Cell culture supernatant", "range": "88 - 98 %", "average": "= 93" }, { "sample": "Serum", "range": "98 - 118 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "117 - 123 %", "average": "= 120" }, { "sample": "Urine", "range": "104 - 110 %", "average": "= 107" }, { "sample": "Heparin Plasma", "range": "100 - 109 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "108 - 116 %", "average": "= 110" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Factor XII also known as FXII or Hageman factor is a serine protease and a coagulation factor that plays an important role in initiating the intrinsic pathway of blood coagulation. It has a molecular mass of approximately 80 kDa. The liver primarily synthesizes this protein and it circulates in the blood plasma as an inactive precursor. When activated to Factor XIIa it cleaves and activates other coagulation factors such as Factor XI to propagate the coagulation cascade that helps in blood clot formation.

Biological function summary

This protein helps control blood coagulation processes as part of a larger network of interacting proteins that includes contact activation factors. Factor XII activates not only the coagulation cascade but also pathways involved in fibrinolysis inflammation and bradykinin formation. It triggers the kallikrein-kinin system and leads to the generation of bradykinin a peptide known to induce vascular permeability and inflammation.

Pathways

The involvement of Factor XII spans the intrinsic coagulation pathway and the kallikrein-kinin system. It interacts with several other coagulation factors such as Factor XI and prekallikrein to advance the clotting process. The protein initiates the intrinsic blood coagulation pathway by converting Factor XI to Factor XIa in the presence of negatively charged surfaces. It links to the fibrinolytic pathway and can indirectly activate plasminogen through interactions that generate plasmin.

Factor XII deficiency leads to increased risk of thrombosis even though such deficiency generally shows no major bleeding symptoms. The target links to hereditary angioedema where dysregulation in the kallikrein-kinin system involves premature bradykinin activity. Factor XII associates with C1-inhibitor a protein playing a regulatory role in preventing excessive bradykinin production which can manifest in clinical symptoms seen in hereditary angioedema patients.

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製品プロトコール

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ターゲットの情報

Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa (PubMed : 2019570, PubMed : 21304106, PubMed : 8427954).

See full target information F12

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of thrombosis and haemostasis : JTH 19:2835-2840 PubMed34363738

2021

Generation of a humanized FXII knock-in mouse-A powerful model system to test novel anti-thrombotic agents.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Beck,David Stegner,Stefan Loroch,Ayesha A Baig,Vanessa Göb,Cornelia Schumbrutzki,Eva Eilers,Albert Sickmann,Frauke May,Marc W Nolte,Con Panousis,Bernhard Nieswandt

Transplantation 103:1821-1833 PubMed30964836

2019

Brain Death Enhances Activation of the Innate Immune System and Leads to Reduced Renal Metabolic Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Laura J Zitur,Peter J Chlebeck,Scott K Odorico,Juan S Danobeitia,Tiffany J Zens,Cees Van Kooten,Michael Eerhart,Jose A Reyes,Megan L Springer,Jennifer M Coonen,Kevin G Brunner,Saverio V Capuano,Anthony M D'Alessandro,Luis A Fernandez

View all publications

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websiteProtocolBooklet

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Human Factor XII ELISA Kit