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AB324325

Human Factor XI ELISA Kit

Human Factor XI ELISA Kit

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Human Factor XI ELISA Kit is a Sandwich ELISA for the measurement of Human Factor XI in Human in Biofluids, Cell Culture Media samples.

別名を表示する

Coagulation factor XI, FXI, Plasma thromboplastin antecedent, PTA, F11

6 Images
Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)

Example of human Factor XI standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)

Interpolated concentration of native Factor XI was measured in duplicate at different sample concentrations. Undiluted samples are 50% HepG2 cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)

Example of human Factor XI standard curve and human Factor XIa NISBC standard (13/100) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)

Interpolated concentration of native Factor XI was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 2,000 human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)

Example of human Factor XI standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)

Interpolated concentration of native Factor XI was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 2,000 human serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 45.115 pg/mL

検出範囲

156.25 - 10000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

Human Factor XI SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Factor XI protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human Factor XI with 45.115 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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精度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.9" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.3" } ]
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回収率

[ { "sample": "Serum", "range": "89 - 94 %", "average": "= 92" }, { "sample": "Citrate plasma", "range": "99 - 112 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "86 - 91 %", "average": "= 88" }, { "sample": "Heparin Plasma", "range": "98 - 112 %", "average": "= 106" }, { "sample": "Cell culture supernatant", "range": "84 - 85 %", "average": "= 85" } ]
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製品内容

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C
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製品プロトコール

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ターゲットの情報

Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.
See full target information F11
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websiteProtocolBooklet
en
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