Human Factor IX/PTC ELISA Kit (ab300307)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 15.884 pg/ml
- Range: 97.656 pg/ml - 6250 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Factor IX/PTC ELISA Kit
Factor IX/PTC キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Serum 8 7.4% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Serum 3 10.1% -
サンプルの種類
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
15.884 pg/ml -
検出範囲
97.656 pg/ml - 6250 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 105 92% - 113% Hep Plasma 112 103% - 118% EDTA Plasma 106 95% - 115% Cit plasma 116 111% - 119% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human Factor IX/PTC SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Factor IX/PTC protein in human serum and plasma. Quantitate Human Factor IX/PTC with 15.884 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 10 x 96 tests 96 tests 1 x 384 tests 10X Human Factor IX/PTC Capture Antibody 1 x 6000µl 1 x 600µl 1 x 600µl 10X Human Factor IX/PTC Detector Antibody 1 x 6000µl 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 200ml 1 x 20ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 0 x 0 unit 1 unit Antibody Diluent 4BI 10 x 6ml 1 x 6ml 1 x 6ml Human Factor IX/PTC Lyophilized Recombinant Protein 2 x 10 vials 2 vials 2 vials Plate Seal 1 x 10 units 1 unit 1 unit Sample Diluent NS (ab193972) 2 x 250ml 1 x 50ml 2 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 x 10 units 1 unit 0 x 0 unit Stop Solution 1 x 120ml 1 x 12ml 2 x 12ml TMB Development Solution 1 x 120ml 1 x 12ml 2 x 12ml -
研究分野
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機能
Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. -
組織特異性
Synthesized primarily in the liver and secreted in plasma. -
関連疾患
Defects in F9 are the cause of recessive X-linked hemophilia B (HEMB) [MIM:306900]; also known as Christmas disease.
Note=Mutations in position 43 (Oxford-3, San Dimas) and 46 (Cambridge) prevents cleavage of the propeptide, mutation in position 93 (Alabama) probably fails to bind to cell membranes, mutation in position 191 (Chapel-Hill) or in position 226 (Nagoya OR Hilo) prevent cleavage of the activation peptide.
Defects in F9 are the cause of thrombophilia due to factor IX defect (THR-FIX) [MIM:300807]. A hemostatic disorder characterized by a tendency to thrombosis. -
配列類似性
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
ドメイン
Calcium binds to the gamma-carboxyglutamic acid (Gla) residues and, with stronger affinity, to another site, beyond the Gla domain. -
翻訳後修飾
Activated by factor XIa, which excises the activation peptide.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Christmas Disease
- Christmas factor
- Coagulant factor IX
see all -
参照データベース
- Entrez Gene: 2158 Human
- Omim: 300746 Human
- SwissProt: P00740 Human
- Unigene: 522798 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300307の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Interpolated concentrations of human Factor IX/PTC in serum in 96-well vs. 384-well plates.
Interpolated concentration of native Factor IX/PTC was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:400 serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Example of human Factor IX/PTC standard curve in Sample Diluent NS in 96-well vs. 384-well plate.Example of human Factor IX/PTC standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human Factor IX/PTC standard curve in Sample Diluent NS.Example of human Factor IX/PTC standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of human Factor IX/PTC in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).Interpolated concentration of native Factor IX/PTC was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 0.15%, plasma (citrate) 0.075%, plasma (EDTA) 0.075%, and plasma (heparin) 0.15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Interpolated concentrations of Factor IX/PTC in normal human serum donors.Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Factor IX/PTC concentration was determined to be 4,867.18 ng/mL with a range of 1,062.16 – 8,981.25 ng/mL.
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Human Factor IX/PTC standard curve comparison.Standard curve comparison between the original Human Factor IX/PTC SimpleStep ELISA (ab188393) and current Human Factor IX/PTC SimpleStep ELISA (ab300307).
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Human Factor IX/PTC serum and plasma comparison.Serum and plasma comparison between the original Human Factor IX/PTC SimpleStep ELISA (ab188393) and current Human Factor IX/PTC SimpleStep ELISA (ab300307).
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Sandwich ELISA - Human Factor IX/PTC ELISA Kit (ab300307)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Human Factor IX/PTC ELISA Kit (ab300307) -
Sandwich ELISA - Human Factor IX/PTC ELISA Kit (ab300307)To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab300307 は論文での使用が確認できていません。