Human Factor IX ELISA Kit

製品の詳細

Human Factor IX ELISA Kit ab108831 is a sandwich ELISA kit to measure Human Factor IX in Serum, Plasma, and Cerebral Spinal Fluid with a sensitivity of 0.33 ng/mL.

How the assay works

Human Factor IX ELISA Kit ab108831 employs a Factor IX specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor IX specific biotinylated detection antibody is added and then followed by washing with wash buffer. StreptavidinPeroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize StreptavidinPeroxidase enzymatic reaction. TMB is catalyzed by StreptavidinPeroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor IX captured in plate.

Assay Specificity

This kit recognizes Factor IX in plasma, serum, and CSF.
This immunoassay is calibrated against a highly purified Human Factor IX. The NIBSC/WHO unclassified purified Human Factor IX preparation 07/182 was evaluated in this kit. The dose response curve of the unclassified standard 07/182 parallels the Factor IX standard curve. To convert sample values obtained with Factor IX Human ELISA Kit (ab46087) to approximate NIBSC Units/mL, use the equation below. NIBSC (07/182) approximate value (Units/mL) = 9029.83 x Factor IX value (ng/mL).

Human Factor IX ELISA Kit ab108831 protocol summary:

1. Add samples and standards to wells. Incubate at room temperature.
2. Wash each well and add biotin-antibody conjugate. Incubate at room temperature.
3. Wash each well and add Streptavidin Solution. Incubate at room temperature.
4. Add Chromogen Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read immediately.

How other researchers are using Human Factor IX ELISA Kit ab108831

Human Factor IX ELISA Kit ab108831 has been used to study targeted overexpression of coagulation factors using CRISPR/Cas9 ⁽¹⁾, the role of Factor IX as a regulator of senescence⁽²⁾, production of coagulation proteins in endothelial cells ⁽³⁾, and charactierizing the coagulation cascade in systemic lupus erythematosus ⁽⁴⁾.

References:

⁽¹⁾ C. Feser, et.al., 2022, PMID: 35563479
⁽²⁾ P. Carpintero-Fernández, et.al., 2022, PMID: 35184131
⁽³⁾ C. Cohen, et.al., 2020, PMID: 32029851

Y. Liang, et.al., 2017, PMID: 29599912

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