Human Factor B ELISA Kit (ab300318)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 18.18 pg/ml
- Range: 53.125 pg/ml - 3400 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Factor B ELISA Kit
Factor B キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Serum 8 4.1% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Serum 3 5.72% -
サンプルの種類
Cell culture supernatant, Saliva, Milk, Urine, Serum, Hep Plasma, EDTA Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
18.18 pg/ml -
検出範囲
53.125 pg/ml - 3400 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 91 86% - 100% Serum 91 88% - 94% Hep Plasma 96 94% - 98% EDTA Plasma 99 92% - 106% Cit plasma 105 103% - 110% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human Factor B SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Factor B protein in human serum, plasma, cell culture supernatant, saliva, urine, and milk. Quantitate Human Factor B with 18.18 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 96 tests 10X Human Factor B Capture Antibody 1 x 600µl 10X Human Factor B Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 96 tests Human Factor B Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 2 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes. -
関連疾患
Defects in CFB are a cause of susceptibility to hemolytic uremic syndrome atypical type 4 (AHUS4) [MIM:612924]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 3 Sushi (CCP/SCR) domains.
Contains 1 VWFA domain. -
細胞内局在
Secreted. - Information by UniProt
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別名
- AHUS4
- B factor properdin
- BF
see all -
参照データベース
- Entrez Gene: 629 Human
- Omim: 138470 Human
- SwissProt: P00751 Human
- Unigene: 69771 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300318の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Example of human Factor B standard curve in Sample Diluent NS.
Example of human Factor B standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of human Factor B in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).Interpolated concentration of native Factor B was measured in duplicate at different sample concentrations. Undiluted samples are 1:100,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Interpolated concentrations of human Factor B in HepG2 cell culture supernatant, saliva, and milk.Interpolated concentration of native Factor B was measured in duplicate at different sample concentrations. Undiluted samples are as follows: HepG2 cell culture supernatant 1:8, saliva 1:16, and milk 1:800. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Interpolated concentrations of Factor B in normal human serum donors.Serum of eight individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Factor B concentration was determined to be 255 µg/mL with a range of 178 – 331 µg/mL.
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Human Factor B ELISA Kit (ab300318) -
Human Factor B ELISA Kit (ab300318)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab300318 は論文での使用が確認できていません。