Human Factor B ELISA Kit (ab137973)
Key features and details
- Sensitivity: 0.98 ng/ml
- Range: 2.188 ng/ml - 140 ng/ml
- Sample type: Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Factor B ELISA Kit
Factor B キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 5.4% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 10.5% -
サンプルの種類
Saliva, Milk, Serum, Plasma, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
>= 0.98 ng/ml -
検出範囲
2.188 ng/ml - 140 ng/ml -
添加回収試験
98 %
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全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Factor B Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human Factor B in plasma, serum, saliva, milk, and cell culture supernatants.
A Factor B specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor B specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor B captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Factor B Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Factor B Microplate (12 x 8 well strips) 1 unit Factor B Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes. -
関連疾患
Defects in CFB are a cause of susceptibility to hemolytic uremic syndrome atypical type 4 (AHUS4) [MIM:612924]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 3 Sushi (CCP/SCR) domains.
Contains 1 VWFA domain. -
細胞内局在
Secreted. - Information by UniProt
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別名
- AHUS4
- B factor properdin
- BF
see all -
参照データベース
- Entrez Gene: 629 Human
- Omim: 138470 Human
- SwissProt: P00751 Human
- Unigene: 69771 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab137973の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Human Complement Factor B typical standard curve
Representative Standard Curve using ab137973
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ELISA: Factor B Human ELISA Kit (ab137973)Standard curve with background signal subtracted (duplicates; +/- SD).
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ELISA: Factor B Human ELISA Kit (ab137973)Factor B measured in culture supernatants (dilution 1/1-1/30; duplicates +/- SD).
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ELISA: Factor B Human ELISA Kit (ab137973)Factor B in biological fluids (duplicates +/- SD). Tested dilution ranges: human plasma and serum at 1/1000-1/30000, others at 1/1-1/300.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab137973 は 5 報の論文で使用されています。
- Bain W et al. Factor H preserves alternative complement function during ARDS, linked to improved survival. ERJ Open Res 9:N/A (2023). PubMed: 37377659
- Cabezas-Falcon S et al. Changes in complement alternative pathway components, factor B and factor H during dengue virus infection in the AG129 mouse. J Gen Virol 102:N/A (2021). PubMed: 33410734
- Cooley LS et al. Experimental and computational modeling for signature and biomarker discovery of renal cell carcinoma progression. Mol Cancer 20:136 (2021). PubMed: 34670568
- Bain W et al. Increased Alternative Complement Pathway Function and Improved Survival during Critical Illness. Am J Respir Crit Care Med 202:230-240 (2020). PubMed: 32374177
- Merchant ML et al. Biomarker enhanced risk prediction for development of AKI after cardiac surgery. BMC Nephrol 19:102 (2018). PubMed: 29720115