Human Endostatin ELISA Kit (ab184864)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7.7 pg/ml
- Range: 32 pg/ml - 2000 pg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Endostatin ELISA Kit
Endostatin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Serum 5 6.9% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Serum 3 7.1% -
サンプルの種類
Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
7.7 pg/ml -
検出範囲
32 pg/ml - 2000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 106 99% - 114% Plasma 105 104% - 107% Cell culture media 88 87% - 88% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human Endostatin ELISA kit (ab184864) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Endostatin protein in human serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Endostatin with 7.7 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits. -
特記事項
Endostatin (20-22 kDa), a cleaved product of the C-terminal domain of collagen XVIII, is an endogenous angiogenesis inhibitor. It was originally found in conditioned media from a murine endothelial tumor cell line, hemangioendothelioma. Endostatin inhibits endothelial cell migration (in vivo and in vitro) and induces endothelial cell apoptosis. It inhibits tumor growth and impairs blood vessel maturation in wound healing. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human Endostatin Capture Antibody 1 x 600µl 10X Human Endostatin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI2 1 x 6ml Human Endostatin Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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関連性
Endostatin (20-22 kDa), a cleaved product of the C-terminal domain of collagen XVIII, is an endogenous angiogenesis inhibitor. It was originally found in conditioned media from a murine endothelial tumor cell line, hemangioendothelioma. Endostatin inhibits endothelial cell migration (in vivo and in vitro) and induces endothelial cell apoptosis. It inhibits tumor growth and impairs blood vessel maturation in wound healing. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes. -
別名
- Alpha 1 collagen type 18 (XVIII)(COL18A1)
- Alpha 1 type XVIII collagen
- Antiangiogenic agent
see all -
参照データベース
- Entrez Gene: 80781 Human
- Omim: 120328 Human
- SwissProt: P39060 Human
関連製品
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ELISA kits
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184864の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Other - Human Endostatin ELISA Kit (ab184864)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Figure 1Example of Endostatin standard curve. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Figure 2aTitration of normal Human serum and plasma samples to within working range of the assay. Background subtracted data from duplicate measurements are plotted.
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Figure 2bTitration of normal Human serum and plasma samples to within working range of the assay. Bar graph denotes quantification of Endostatin interpolated from standard curve and multiplied by dilution factor.
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Figure 3Titration of individual donor serum samples to within working range of the assay. Quantification of Endostatin interpolated from standard curve and multiplied by dilution factor.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab184864 は論文での使用が確認できていません。