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リコンビナントSimpleStep

Human DLL3 ELISA Kit (ab300312)

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Example of human DLL3 standard curve in 1X Cell Extraction Buffer PTR.
  • Interpolated concentrations of human DLL3 in cell extract.
  • Human DLL3 ELISA Kit (ab300312)
  • Human DLL3 ELISA Kit (ab300312)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 42.301 pg/ml
  • Range: 54.688 pg/ml - 3500 pg/ml
  • Sample type: Cell Lysate
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

製品の概要

  • 製品名

    Human DLL3 ELISA Kit
    DLL3 キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Extract 8 3.1%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Extract 3 7%

  • サンプルの種類

    Cell Lysate

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    42.301 pg/ml

  • 検出範囲

    54.688 pg/ml - 3500 pg/ml

  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell Lysate 101 97% - 105%

  • 全工程の試験時間

    1h 30m

  • ステップ

    One step assay

  • 種交差性

    交差種: Human

  • 製品の概要

    Human DLL3 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DLL3 protein in human cell extract. Quantitate Human DLL3 with 42.301 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


    -Single-wash protocol reduces assay time to 90 minutes or less
    -High sensitivity, specificity and reproducibility from superior antibodies
    -Fully validated in biological samples
    -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • 特記事項

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 96 tests
    10X Human DLL3 Capture Antibody 1 x 600µl
    10X Human DLL3 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BI 1 x 6ml
    Human DLL3 Lyophilized Recombinant Protein 2 vials
    Plate Seal 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • 研究分野

  • 機能

    Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm.

  • 関連疾患

    Spondylocostal dysostosis 1

  • 配列類似性

    Contains 1 DSL domain.
    Contains 6 EGF-like domains.

  • ドメイン

    The DSL domain is required for binding to the Notch receptor.

  • 翻訳後修飾

    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 別名

    • Delta Drosophila like 3
    • Delta like 3 Drosophila
    • Delta like 3 homolog Drosophila
    • Delta like 3 protein
    • Delta like protein 3 precursor
    • Delta-like protein 3
    • Delta3
    • Dll3
    • DLL3_HUMAN
    • Drosophila Delta homolog 3
    • SCDO1
    • SCOD1
    • Spondylocostal dysostosis autosomal recessive
    see all

  • 参照データベース

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab300312の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Sandwich ELISA
    Use at an assay dependent concentration.
    特記事項
    Sandwich ELISA
    Use at an assay dependent concentration.

    画像

    • Example of human DLL3 standard curve in 1X Cell Extraction Buffer PTR.
      Example of human DLL3 standard curve in 1X Cell Extraction Buffer PTR.

      Example of human DLL3 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of human DLL3 in cell extract.
      Interpolated concentrations of human DLL3 in cell extract.

      Interpolated concentration of native DLL3 was measured in duplicate at different sample concentrations. Undiluted samples are 20 µg/mL TT cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

    • Human DLL3 ELISA Kit (ab300312)
      Human DLL3 ELISA Kit (ab300312)
    • Human DLL3 ELISA Kit (ab300312)
      Human DLL3 ELISA Kit (ab300312)

    参考文献 (0)

    ab300312 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300312 は論文での使用が確認できていません。

    レビューと Q&A

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