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AB324139

Human DKKL1 ELISA Kit

Human DKKL1 ELISA Kit

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Human DKKL1 ELISA Kit is a Sandwich ELISA for the measurement of Human DKKL1 in Human in Cell/Tissue Extracts samples.

別名を表示する

SGY1, UNQ735/PRO1429, DKKL1, Dickkopf-like protein 1, Cancer/testis antigen 34, Protein soggy-1, CT34, SGY-1

2 Images
Sandwich ELISA - Human DKKL1 ELISA Kit (AB324139)
  • sELISA

Supplier Data

Sandwich ELISA - Human DKKL1 ELISA Kit (AB324139)

Example of human DKKL1 standard curve 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human DKKL1 ELISA Kit (AB324139)
  • sELISA

Supplier Data

Sandwich ELISA - Human DKKL1 ELISA Kit (AB324139)

Interpolated concentration of native DKKL1 was measured in duplicate at different sample concentrations. Undiluted samples are 500 ug/mL human testes tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell Lysate, Tissue Extracts

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 5.191 pg/mL

検出範囲

18.75 - 1200 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

Human DKKL1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DKKL1 protein in Cell Lysate, Tissue extracts. Quantitate Human DKKL1 with 5.191 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

  • Singlewash protocol reduces assay time to 90 minutes or less.
  • High sensitivity, specificity and reproducibility from superior antibodies.
  • Fully validated in biological samples.
  • 96wells plate breakable into 12 x 8 wells strips, available in 10pack (10 x 96well plates).

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.8" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.7" } ]
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回収率

[ { "sample": "Cell Lysate", "range": "92 - 95 %", "average": "= 93" }, { "sample": "Tissue Extracts", "range": "88 - 99 %", "average": "= 94" } ]
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製品内容

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C
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製品プロトコール

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ターゲットの情報

Involved in fertilization by facilitating sperm penetration of the zona pellucida. May promote spermatocyte apoptosis, thereby limiting sperm production. In adults, may reduce testosterone synthesis in Leydig cells. Is not essential either for development or fertility.
See full target information DKKL1
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websiteProtocolBooklet
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Abcam product promise

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