Human Anti-Dengue virus IgG ELISA Kit (ab108728)

The Dengue IgG and IgM Kits have been validated for use on serum samples and plasma samples collected with citrate. Although it may well work we have not tried the kit with samples collected using EDTA. We would therefore not be able to guarantee the performance of the kit with these samples.

If you would like to purchase either ab108728 or ab108729 and use it with your serum prepared with EDTA, you would be eligible to participate in the Abtrial scheme. This offer means that if you purchase ab108728 or ab108729 as normal, test the kit with your samples in and let us know of the results through an Abreview (no matter whether positive or negative) you would then be eligible to claim the value of ab108728 or ab108729 off any product in our catalogue. More information on this offer can be found here:

www.abcam.com/abtrial

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab108728 or ab108729.

I contacted the laboratory, and they confirmed that ab108728 does in fact cross react with Dengue Virus serotypes 1-4. It is very difficult to get samples with defined subtype of Dengue because the antibodies show a strong cross reactivity. Due to this cross reactivity it is sufficient to use Dengue-2 antigen (the antigen used in ab108728) to detect antibodies against subtypes 1-4. I have attached an extract of a WHO publication. On page 100 of the original document you will find the related statement in the paragraph right above the figure.

This product is not in stock and currently has a 1 -2 week lead time. The kit will recognize all four serotypes (DEN-1, DEN-2, DEN-3, and DEN-4). While the Dengue Virus IgG ELISA is coated with purified Dengue 2 Virus proteins from strain 16681. Dengue Virus Type 2 is cross reactive with antibodies from Serotypes 1-4.

The Dengue Virus IgG ELISA is coated with purified Dengue 2 Virus proteins from strain 16681. Dengue Virus Type 2 is cross reactive with antibodies from Serotypes 1-4, thus the kit will detect IgG from patient samples past infected with any of the four serotypes. The kit is not specific to Dengue Type 2.

It has not been tested for reactivity to west nile virus.

In this assay, microtiter strip wells are precoated with Dengue Virus antigens type 2 to bind corresponding antibodies of the specimen. No capture antibody is used. The only antibody in the kit is the anti-human-HRP conjugate used to detect any antibody bound to the Dengue Virus antigens type 2.

We do not have cross reactivity data for West Nile Virus.

Te pido disculpas por la espera. Tenemos en el catálogo dos kits para determinar el virus Dengue en función del isotipo. Éstos son: ab108728 y ab108729. En ambos kits las placas están cubiertas con antígenos de virus 2 de la cepa 16681. Espero haberte ayudado. No dudes en contactarnos para cualquier otra duda.