Human DcR1 ELISA Kit (CD263) (ab46016)
Key features and details
- Sensitivity: 147 pg/ml
- Range: 312.5 pg/ml - 10000 pg/ml
- Sample type: Cell Lysate, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human DcR1 ELISA Kit (CD263) -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Sample A 8 7171pg/ml 192 2.7% Sample B 8 3584pg/ml 244 6.8% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Sample A 9 7311pg/ml 515 7% Sample B 9 2654pg/ml 356 9.7% -
サンプルの種類
Serum, Cell Lysate -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 147 pg/ml -
検出範囲
312.5 pg/ml - 10000 pg/ml -
全工程の試験時間
3h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s DcR1 (CD263) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of DcR1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
A monoclonal antibody specific for DcR1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known DcR1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for DcR1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of DcR1 present in the samples.
This kit will recognize both endogenous and recombinant Human DcR1.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml Biotinylated anti-DcR1 Red 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml DcR1 Microplate (12 x 8 well strips) 1 unit 2 units DcR1 Standard (Lyophilized) Yellow 2 vials 4 vials HRP Diluent Red 1 x 12ml 1 x 23ml Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究分野
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関連性
DcR1 is a member of the TNF-receptor superfamily. It contains an extracellular TRAIL-binding domain and a transmembrane domain, but no cytoplasmic death domain. It is not capable of inducing apoptosis, and is thought to function as an antagonistic receptor that protects cells from TRAIL-induced apoptosis. This gene was found to be a p53-regulated DNA damage-inducible gene. The expression of this gene was detected in many normal tissues but not in most cancer cell lines, which may explain the specific sensitivity of cancer cells to the apoptosis-inducing activity of TRAIL. -
細胞内局在
Cell membrane; lipid-anchor; GPI-anchor -
別名
- CD263
- DCR1
- Decoy receptor 1
see all -
参照データベース
- Entrez Gene: 8794 Human
- SwissProt: O14798 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46016の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Typical Standard Curve
Representative Standard Curve using ab46016
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab46016 は 1 報の論文で使用されています。
- Anand A et al. Does DcR1 (TNF-related apoptosis-inducing-ligand Receptor 3) have any role in human AMD pathogenesis? Sci Rep 4:4114 (2014). PubMed: 24534820