AB317544

Human DAP12 ELISA Kit

SimpleStep
Recombinant
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Human DAP12 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human DAP12 with a sensitivity of 2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

DAP12, KARAP, TYROBP, TYRO protein tyrosine kinase-binding protein, DNAX-activation protein 12, Killer-activating receptor-associated protein, KAR-associated protein

4 Images

Sandwich ELISA - Human DAP12 ELISA Kit (AB317544)

sELISA

Supplier Data

Sandwich ELISA - Human DAP12 ELISA Kit (AB317544)

Interpolated concentrations of human DAP12 in THP-1 cell extract

Interpolated concentration of native DAP12 was measured in duplicate at different sample concentrations. Undiluted samples are 60 µg/mL THP-1 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human DAP12 ELISA Kit (AB317544)

Interpolated concentrations of human DAP12 in THP-1 cell extract in 96-well vs. 384-well plates

Interpolated concentration of native DAP12 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 60 µg/mL THP-1 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human DAP12 ELISA Kit (AB317544)

Example of human DAP12 standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human DAP12 ELISA Kit (AB317544)

Example of human DAP12 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell Lysate, Tissue Extracts

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 2 pg/mL

検出範囲

15.625 - 1000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human DAP12 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DAP12 protein in Cell Lysate, Tissue extracts. Quantitate Human DAP12 with 2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" } ]

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回収率

[ { "sample": "Cell Lysate", "range": "95 - 109 %", "average": "= 103" }, { "sample": "Tissue Extracts", "range": "92 - 106 %", "average": "= 97" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Adapter protein which non-covalently associates with activating receptors found on the surface of a variety of immune cells to mediate signaling and cell activation following ligand binding by the receptors (PubMed : 10604985, PubMed : 9490415, PubMed : 9655483). TYROBP is tyrosine-phosphorylated in the ITAM domain following ligand binding by the associated receptors which leads to activation of additional tyrosine kinases and subsequent cell activation (PubMed : 9490415). Also has an inhibitory role in some cells (PubMed : 21727189). Non-covalently associates with activating receptors of the CD300 family to mediate cell activation (PubMed : 15557162, PubMed : 16920917, PubMed : 17928527, PubMed : 26221034). Also mediates cell activation through association with activating receptors of the CD200R family (By similarity). Required for neutrophil activation mediated by integrin (By similarity). Required for the activation of myeloid cells mediated by the CLEC5A/MDL1 receptor (PubMed : 10449773). Associates with natural killer (NK) cell receptors such as KIR2DS2 and the KLRD1/KLRC2 heterodimer to mediate NK cell activation (PubMed : 23715743, PubMed : 9490415, PubMed : 9655483). Also enhances trafficking and cell surface expression of NK cell receptors KIR2DS1, KIR2DS2 and KIR2DS4 and ensures their stability at the cell surface (PubMed : 23715743). Associates with SIRPB1 to mediate activation of myeloid cells such as monocytes and dendritic cells (PubMed : 10604985). Associates with TREM1 to mediate activation of neutrophils and monocytes (PubMed : 10799849). Associates with TREM2 on monocyte-derived dendritic cells to mediate up-regulation of chemokine receptor CCR7 and dendritic cell maturation and survival (PubMed : 11602640). Association with TREM2 mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages (PubMed : 18957693). Stabilizes the TREM2 C-terminal fragment (TREM2-CTF) produced by TREM2 ectodomain shedding which suppresses the release of pro-inflammatory cytokines (PubMed : 25957402). In microglia, required with TREM2 for phagocytosis of apoptotic neurons (By similarity). Required with ITGAM/CD11B in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity). Promotes pro-inflammatory responses in microglia following nerve injury which accelerates degeneration of injured neurons (By similarity). Positively regulates the expression of the IRAK3/IRAK-M kinase and IL10 production by liver dendritic cells and inhibits their T cell allostimulatory ability (By similarity). Negatively regulates B cell proliferation (PubMed : 21727189). Required for CSF1-mediated osteoclast cytoskeletal organization (By similarity). Positively regulates multinucleation during osteoclast development (By similarity).

See full target information TYROBP

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websiteProtocolBooklet

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Human DAP12 ELISA Kit