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Human Anti-Cytomegalovirus IgG ELISA Kit (CMV) (ab108724)

Reviews (2)Q&A (5)References (16)

Key features and details

  • Sample type: Cit plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Indirect
  • Reacts with: Human

製品の概要

  • 製品名

    Human Anti-Cytomegalovirus IgG ELISA Kit (CMV)

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Pos.Serum 12 1.9%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Pos.Serum 4 6.2%

  • サンプルの種類

    Serum, Hep Plasma, Cit plasma

  • アッセイタイプ

    Indirect

  • ステップ

    Multiple steps standard assay

  • 種交差性

    交差種: Human

  • 製品の概要

    Abcam’s anti-Cytomegalovirus (CMV) IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the qualitative measurement of IgG class antibodies against Cytomegalovirus in Human serum and plasma.

     

    A 96-well plate has been precoated with Cytomegalovirus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Cytomegalovirus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Cytomegalovirus IgG sample captured in plate.

  • アプリケーション

    適用あり: ELISAmore details

  • 試験プラットフォーム

    Microplate

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108724の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
特記事項
ELISA
Use at an assay dependent concentration.

参考文献 (16)

ab108724 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108724 は 16 報の論文で使用されています。

  • Capelle CM  et al. Early-to-mid stage idiopathic Parkinson's disease shows enhanced cytotoxicity and differentiation in CD8 T-cells in females. Nat Commun 14:7461 (2023). PubMed: 37985656
  • Zeng N  et al. DJ-1 depletion prevents immunoaging in T-cell compartments. EMBO Rep 23:e53302 (2022). PubMed: 35037711
  • Sharpe HR  et al. CMV-associated T cell and NK cell terminal differentiation does not affect immunogenicity of ChAdOx1 vaccination. JCI Insight 7:N/A (2022). PubMed: 35192547
  • Alanio C  et al. Cytomegalovirus Latent Infection is Associated with an Increased Risk of COVID-19-Related Hospitalization. J Infect Dis 226:463-473 (2022). PubMed: 35134186
  • Kamori D  et al. Seroprevalence of Human Herpesvirus Infections in Newly Diagnosed HIV-Infected Key Populations in Dar es Salaam, Tanzania. Int J Microbiol 2021:4608549 (2021). PubMed: 34484345
View all Publications for this product

レビューと Q&A

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1-7 of 7 Abreviews or Q&A

Tested against citrate plasma vs. EDTA plasma

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
Blood from the same donor was collected into both citrate and EDTA collection tubes for direct comparison of citrate vs. EDTA plasma. ELISA was performed according to the manufacturer's instructions including substrate blank, negative control, cut-off control, positive control, and duplicates for EDTA plasma and citrate plasma. As shown in the figure, EDTA plasma and citrate plasma had comparable absorbance results.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 04 2023

Quantification of IgG anti-CMV ELISA in frozen plasma samples with ab108724 Anti-cytomegalovirus (CMV) IgG human ELISA kit

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
Quantification of human IgG anti-CMV in EDTA plasma samples (freshly frozen and stored at -80C) was tested using the ab108724 ELISA kit. The information provided with the kit stated that only serum samples and citrate plasma samples had been previously tested. We wanted to test whether the kit allowed accurate quantification of human IgG anti-CMV in EDTA plasma.
Plasma samples were diluted 1:100 with the sample diluent provided with the kit (as per the manufacturer’s instructions) and assayed in duplicate. Duplicate wells for positive control, cut-off and negative control as well as blank were included as per the protocol provided. All steps followed the instructions provided with the ELISA kit.
Absorbance (optical density, OD) was determined with a plate reader set at 450 nm with a reference wavelength of 650 nm. Absorbance of blank wells was averaged and subtracted from all other wells. Absorbance values were converted into standard units as instructed by the manufacturer (cut-off = 10 units positive > 11 units negative < 9 units grey zone = 9-11 units).
The kit distinguished clearly between IgG anti-CMV positive and negative samples and allowed quantification of these antibodies in EDTA plasma samples (see Figure).
The protocol provided with the kit was clear and easy to follow and contained all the information necessary to perform the assay.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 04 2019

Question

Do you sell just the antigen for the below and if so at what cost?

Adenovirus IgG Human ELISA Kit (ab108705)
Measles Virus IgG Human ELISA Kit (ab108750)
Cytomegalovirus IgG (CMV IgG) Human ELISA Kit (ab108724)
Epstein-Barr Virus (EBNA) IgG Human ELISA Kit (ab108731)

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Answer

Thank you for your email. I do apologize for the delay in responding to your email.

We unfortunately do not sell the antigens for these kits seperately.

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Question

We would be interested in obtaining a quote for the ToRCH range of assays. In particular:


Toxoplasma IgG

Toxoplasma IgM

Rubella IgG

Rubella IgM

CMV IgG

CMV IgM

HSV-1 IgG

HSV-2 IgG

HSV-1 & 2 IgM


We look forward to hearing from you soon.

Kind regards,

Read More
Answer

Thank you for your email.

The product I have identified for you are as follows;

ab108776
ab108778

ab108767
we do not have anti rubella IgM kit

ab108724
ab108725

ab108737
ab108738

ab108739
ab108740

ab108742

I have contacted my colleague who will give you a bulk quote for this kit. Please let me know if you do not hear from us within 24 hours of this email.

I hope this information will be helpful. Should you require more information please do not hesitate to contact me.

Read More

Question

Interested in finding out which antigen is coated on ab108724. Is working on mouse CMV and wants to know what antigens human kits employ

Read More
Answer

Thank you for your inquiry.



I am happy to confirm that the plates of ab108724 is coated with CMV Strain AD-169 Antigens.

I hope this information is helpful. Please do not hesitate to contact em again with any further questions.

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Question

I have a few questions about ab108724? 1. What is the detection limit? 2. What are "cut off" controls? 3. How are controls to be prepared?

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Answer

A1: The CMV ELISA, ab108724, is qualitative and therefore does not have a known detection limit. The assay sensitivity is defined as the probability of the assay scoring positive in the presence of specific analyte. The assay sensitivity for this kit is 98%. A2: The cut-off control is used to determine the optical density threshold at which samples are considered positive or negative. Please see the product insert for details on this calculation. A3: The controls in this kit come in liquid form ready to use.

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Question

 I have some questions to ask regarding CMV IgG Human ELISA Kit # ab108724. It would be really appreciated if you could provide me answers to the following questions. 1.       What is the limit of detection and specificity of the assay? 2.       Please provide some guidance on spiking experiments? 3.       Any recommendation for heat-denaturing of serum to mitigate albumin interference? 4.       Any known non-specific binding concerns that we can address to this assay?  

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Answer

Thank you for your patience. The laboratory that developed the ELISA has sent the following information. If it does not completely answer your questions, please contact me. The CMV IgG ELISA is a qualitative test. Therefore we do not have data about the detection limit or spiking. Heat inactivation of samples is not recommended since it influences the test results in a negative way. Cross reactivity with antibodies against HSV1-2, Measles, Mumps, or VZV have not been observed. The diagnostics specificity is defined as the probability of the assay scoring negative in the absence of specific analyte. For this, it is 97.5%. The diagnostic senstivity is defined as the probability of the assay scoring positive in the presence of specific analyte. The sensitivity of this assay is 98%. Interferences with hemolytic, lipemic, or icteric sera are not ovserved up to a concentration of 10 mg/ml hemoglobin, 5 mg/ml triglycerides, and 0.2 mg/ml bilirubin.  

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