Human Cytokeratin 5 ELISA Kit

製品の詳細

Human Cytokeratin 5 ELISA Kit (ab226896) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cytokeratin 5 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cytokeratin 5 with 129 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Cytokeratin 5 is an intermediate filament protein in the keratin family and is a component of the cytoskeletal scaffold in epithelial cells. Cytokeratin 5 is mutated in epidermolysis bullosa simplex. Cytokeratin 5 is also a candidate biomarker for lung and breast cancers. Cytokeratin 5 is a 590 residue protein and the antibodies in this kit were raised to the C-terminal 250 residues.