AB254515

Human Cytokeratin 18 Fragment ELISA Kit (CK18-30)

Recombinant
SimpleStep
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Human Cytokeratin 18 Fragment ELISA Kit (CK18-30) is a single-wash 90-min Simplestep used to quantify Human Cytokeratin 18 Fragment (CK18-30) with a sensitivity of 22.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18

5 Images

ELISA - Human Cytokeratin 18 Fragment ELISA Kit (CK18-30) (AB254515)

ELISA

Supplier Data

ELISA - Human Cytokeratin 18 Fragment ELISA Kit (CK18-30) (AB254515)

sELISA

Supplier Data

Sandwich ELISA - Human Cytokeratin 18 Fragment ELISA Kit (CK18-30) (AB254515)

Interpolated concentrations of native Cytokeratin 18 Fragment (CK18-30) in human HeLa cell extract and extract based on a 12.5 μg/mL and 1.56 µg/mL extract load (respectively).

The concentrations of Cytokeratin 18 Fragment were measured in duplicate and interpolated from the Cytokeratin 18 Fragment standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 18 Fragment concentration was determined to be 42.1 pg/mL / μg/mL in untreated and 394 pg/mL / μg/mL in STS treated HeLa cell extract.

sELISA

Supplier Data

Sandwich ELISA - Human Cytokeratin 18 Fragment ELISA Kit (CK18-30) (AB254515)

Example of human Cytokeratin 18 Fragment (CK18-30) standard curve in Sample Diluent NS.

The Cytokeratin 18 Fragment standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Cytokeratin 18 Fragment ELISA Kit (CK18-30) (AB254515)

Interpolated concentrations of spike Cytokeratin 18 Fragment (CK18-30) in human serum and plasma samples.

The concentrations of Cytokeratin 18 Fragment were measured in duplicates, interpolated from the Cytokeratin 18 Fragment standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma – citrate 25%, plasma- EDTA 25%, and plasma – heparin 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Cytokeratin 18 Fragment ELISA Kit (CK18-30) (AB254515)

Interpolated concentrations of native Cytokeratin 18 Fragment (CK18-30) in human HeLa cell culture supernatant samples.

The concentrations of Cytokeratin 18 Fragment were measured in duplicates, interpolated from the Cytokeratin 18 Fragment standard curves and corrected for sample dilution. Undiluted samples are as follows : untreated 100% and staurosporine treated (4hr at 1 μM) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 18 Fragment concentration was determined to be 239 pg/mL in untreated and 400 pg/mL in STS treated cell culture supernatant.

Key facts

検出方法

Colorimetric

サンプルタイプ

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 22.8 pg/mL

検出範囲

46.875 - 3000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human Cytokeratin 18 Fragment ELISA Kit (CK18-30) ab254515 is a rapid single-wash 90-min Sandwich ELISA to measure Human Cytokeratin 18 Fragment (CK18-30)in cell culture supernatant, cell lysate, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 22.8 pg/mL.

How the assay works

Human Cytokeratin 18 Fragment (CK18-30)SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Cytokeratin 18 Fragment ELISA Kit (CK18-30) ab254515 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Cytokeratin 18 Fragment is a degradation product of Cytokeratin 18. Cytokeratin 18 is involved in the uptake of thrombin-antithrombin complexes by hepatic cells. Cytokeratin 18 becomes cleaved during apoptosis resulting in a 18kDa fragment, which can be used as a measure of cell death and a biomarker for liver disease. ab254515 recognizes this fragment, and specifically recognizes the cleavage site (M30) neo-epitope and does not recognize the full-length protein.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.5" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" } ]

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回収率

[ { "sample": "Cell culture supernatant", "range": null, "average": "= 97" }, { "sample": "Serum", "range": null, "average": "= 87" }, { "sample": "EDTA Plasma", "range": null, "average": "= 85" }, { "sample": "Heparin Plasma", "range": null, "average": "= 79" }, { "sample": "Citrate plasma", "range": null, "average": "= 84" }, { "sample": "Cell Lysate", "range": null, "average": "= 87" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

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製品プロトコール

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ターゲットの情報

Required for the formation of KRT8/KRT18 filaments that are involved in ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber formation and reinforcement (PubMed : 26823019). Also acts downstream of ROCK kinase activation as part of a positive feedback mechanism in response to cellular mechanical stress loading (PubMed : 26823019). Organization and orientation of KRT18 filaments are responsible for the properly elongated morphology of epithelial tubules (By similarity). Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

See full target information KRT18

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Biomedical reports 22:60 PubMed39991001

2025

Scutellarein enhances cisplatin‑induced apoptotic effects by suppressing the PI3K/AKT‑MDR1 pathway in human NPC/HK1 nasopharyngeal carcinoma cells.

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Yen-Ting Wu,Lei-Chin Chen,Han-Hsuan Wang,Chen-Xuan He,Chia-Hung Chien,Chih-Chieh Tao,Yingxiao Li,Yun-Ju Chen,Yu-Yan Lan

Biomedical reports 21:143 PubMed39161943

2024

Treatment with autophagic inhibitors enhances oligonol‑induced apoptotic effects in nasopharyngeal carcinoma cells.

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Yen-Ting Wu,Cheng-Han Lin,Wen-Chin Chiu,Tsung-Jen Hsieh,Sue-Joan Chang,Yun-Ching Chang,Yu-Yan Lan

Evidence-based complementary and alternative medicine : eCAM 2022:1842363 PubMed38023774

2022

Cordycepin Induces Apoptosis through JNK-Mediated Caspase Activation in Human OEC-M1 Oral Cancer Cells.

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Kuo-Lung Tung,Su-Zhen Wu,Chun-Chuan Yang,Hong-Yi Chang,Chun-Sheng Chang,Yan-Hsiung Wang,Bu-Miin Huang,Yu-Yan Lan

View all publications

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websiteProtocolBooklet

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Human Cytokeratin 18 Fragment ELISA Kit (CK18-30)