JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB323523

Human CXCR4 ELISA Kit- Extracellular

Human CXCR4 ELISA Kit- Extracellular

Be the first to review this product! Submit a review

|

(0 Publication)

Human CXCR4 ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human CXCR4 with a sensitivity of 5 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

CD184, C-X-C chemokine receptor type 4, CXC-R4, CXCR-4, FB22, Fusin, HM89, LCR1, Leukocyte-derived seven transmembrane domain receptor, Lipopolysaccharide-associated protein 3, NPYRL, Stromal cell-derived factor 1 receptor, LESTR, LAP-3, LPS-associated protein 3, SDF-1 receptor, CXCR4

2 Images
Sandwich ELISA - Human CXCR4 ELISA Kit- Extracellular (AB323523)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCR4 ELISA Kit- Extracellular (AB323523)

Example of human CXCR4 standard curve in Sample Diluent NS + 1X Enhancer in 96-well vs. 384-well plate.

Example of human CXCR4 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CXCR4 ELISA Kit- Extracellular (AB323523)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCR4 ELISA Kit- Extracellular (AB323523)

Example of human CXCR4 standard curve in Sample Diluent NS + 1X Enhancer.

Example of human CXCR4 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 5 pg/mL

検出範囲

28.125 - 1800 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

製品の詳細

Human CXCR4 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCR4 protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media. Quantitate Human CXCR4 with 5 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

  • Single-wash protocol reduces assay time to 90 minutes or less
  • High sensitivity, specificity and reproducibility from superior antibodies
  • Fully validated in biological samples
  • 96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
  • Also available in a fully validated 384-well format.
style
left-column

精度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.4" } ]
style
left-column

回収率

[ { "sample": "Citrate plasma", "range": "82 - 91 %", "average": "= 87" }, { "sample": "EDTA Plasma", "range": "76 - 90 %", "average": "= 84" }, { "sample": "Heparin Plasma", "range": "86 - 92 %", "average": "= 88" }, { "sample": "Cell culture media", "range": "92 - 97 %", "average": "= 94" } ]
style
left-column

製品内容

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab323523-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB323523-CMP04" }, { "size":"20 x 1 Vial", "name":"Human CXCR4 Lyophilized Recombinant Protein", "number":"AB323523-CMP09" }, { "size":"10 x 600 µL", "name":"10X Human CXCR4 Detector Antibody", "number":"AB323523-CMP08" }, { "size":"10 x 600 µL", "name":"10X Human CXCR4 Capture Antibody", "number":"AB323523-CMP01" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323523-CMP14" }, { "size":"10 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB323523-CMP12" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB323523-CMP02" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB323523-CMP03" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB323523-CMP06" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB323523-CMP05" }, { "size":"10 x 500 µL", "name":"Denaturant", "number":"AB323523-CMP11" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB323523-CMP10" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab323523-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB323523-CMP04" }, { "size":"2 x 1 Vial", "name":"Human CXCR4 Lyophilized Recombinant Protein", "number":"AB323523-CMP09" }, { "size":"1 x 600 µL", "name":"10X Human CXCR4 Detector Antibody", "number":"AB323523-CMP08" }, { "size":"1 x 600 µL", "name":"10X Human CXCR4 Capture Antibody", "number":"AB323523-CMP01" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323523-CMP14" }, { "size":"1 x 5 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB323523-CMP13" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB323523-CMP02" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB323523-CMP03" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB323523-CMP06" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB323523-CMP07" }, { "size":"1 x 500 µL", "name":"Denaturant", "number":"AB323523-CMP11" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB323523-CMP10" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab323523-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB323523-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CXCR4 Lyophilized Recombinant Protein", "number":"AB323523-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CXCR4 Detector Antibody", "number":"AB323523-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CXCR4 Capture Antibody", "number":"AB323523-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323523-CMP14", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB323523-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB323523-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB323523-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB323523-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB323523-CMP05", "productcode":"" }, { "size":"1 x 500 µL", "name":"Denaturant", "number":"AB323523-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB323523-CMP10", "productcode":"" } ] } } }
style
left-column

出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C
style
left-column
style
left-column

製品プロトコール

style
left-column

ターゲットの情報

Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation (PubMed : 10074102, PubMed : 10452968, PubMed : 10644702, PubMed : 10825158, PubMed : 18799424, PubMed : 20048153, PubMed : 20505072, PubMed : 24912431, PubMed : 28978524, PubMed : 8752280, PubMed : 8752281). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed : 16725153, PubMed : 17197449, PubMed : 18799424, PubMed : 39093700). CXCR4 is coupled to G(i) G alpha proteins and mediates inhibition of adenylate cyclase (PubMed : 17197449, PubMed : 39093700). Involved in the AKT signaling cascade (PubMed : 24912431). Plays a role in regulation of cell migration, e.g. during wound healing (PubMed : 28978524). Also acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels (PubMed : 20228059). Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed : 11276205). Involved in hematopoiesis and in cardiac ventricular septum formation (By similarity). Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells (By similarity). Involved in cerebellar development; in the CNS, could mediate hippocampal-neuron surviva (By similarity).. (Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) for human immunodeficiency virus-1/HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus (PubMed : 10074122, PubMed : 10756055, PubMed : 8849450, PubMed : 8929542, PubMed : 9427609).
See full target information CXCR4
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com