Human CXCL5 ELISA Kit

• sELISA

Supplier Data

Sandwich ELISA - Human CXCL5 ELISA Kit (AB212163)

Plasma from three individual healthy human donors (pooled gender) was collected and centrifuged for 15 minutes at 1,000 x g.

Half of the volume of each sample was then additionally centrifuged at 10,000 x g for 10 minutes at 4^oC to remove platelets (platelet poor). All plasma samples were diluted 1 : 2 in Sample Diluent NS and measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL5 concentration was determined to be 825 pg/mL in plasma (citrate), 852.0 pg/mL in platelet poor plasma (citrate), 255 pg/mL in plasma (EDTA), 254.2 pg/mL in platelet poor plasma (EDTA), 1,067.8 pg/mL in plasma (heparin), and 1,065.2 pg/mL in platelet poor plasma (heparin).

• sELISA

Supplier Data

Sandwich ELISA - Human CXCL5 ELISA Kit (AB212163)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL5 concentration was determined to be 641.9 pg/mL with a range of 14.1 – 1,315.6 pg/mL.

• sELISA

Supplier Data

Sandwich ELISA - Human CXCL5 ELISA Kit (AB212163)

Interpolated concentrations of native CXCL5 in human serum and plasma samples.

The concentrations of CXCL5 were measured in duplicates, interpolated from the CXCL5 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 50%, plasma (EDTA) 50% and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL5 concentration was determined to be 622.1 pg/mL in serum, 1,977.3 pg/mL in plasma (citrate), 511.4 pg/mL in plasma (EDTA) and 706.2 pg/mL in plasma (heparin).

• sELISA

Supplier Data

Sandwich ELISA - Human CXCL5 ELISA Kit (AB212163)

Interpolated concentrations of native CXCL5 in human PBMC cell culture supernatant samples from two different donors. All samples were diluted 1 : 40 and the concentrations of CXCL5 were measured in duplicates, interpolated from the CXCL5 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL5 concentration was determined to be 1,188.3 pg/mL in PBMC unstimulated supernatant (Donor 1), 2,702.2 pg/mL in stimulated PBMC supernatant (Donor 1), 567.2 pg/mL in PBMC unstimulated supernatant (Donor 2), and 21,325.6 pg/mL in stimulated PBMC supernatant (Donor 2). PBMC were cultured in RPMI media with 10% fetal bovine serum and 1% PenStrep in the absence (unstimulated) or presence (stimulated) of 1.5% PHA-M for 48 hours.

• sELISA

Supplier Data

Sandwich ELISA - Human CXCL5 ELISA Kit (AB212163)

Interpolated concentrations of native CXCL5 in human PHA-M stimulated PBMC cell culture supernatant samples from two different donors. The concentrations of CXCL5 were measured in duplicates, interpolated from the CXCL5 standard curves and corrected for sample dilution. Undiluted samples are PHA-M stimulated PBMC supernatant (Donor 1) 10% and PHA-M stimulated PBMC supernatant (Donor 2) 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL5 concentration was determined to be 2,702.1 pg/mL in PBMC supernatant (Donor 1), and 21,336.6 pg/mL in PBMC supernatant (Donor 2).

• sELISA

Unknown

Sandwich ELISA - Human CXCL5 ELISA Kit (AB212163)

Human CXCL5 standard curve comparison data.

Standard curve comparison between human CSCL5 SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 6-fold increase in sensitivity.

• sELISA

Supplier Data

Sandwich ELISA - Human CXCL5 ELISA Kit (AB212163)

Example of human CXCL5 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.