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SimpleStep

Human CXCL16 ELISA Kit (ab187397)

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Other - Human CXCL16 ELISA Kit (ab187397)
  • Example of CXCL16 standard curve.
  • Titration of Human serum and plasma within the working range of the assay.
  • Titration of HepG2 cell culture supernatant within the working range of the assay.
  • CXCL16 concentrations in 10 individual Human serum donors.

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 2 pg/ml
  • Range: 31.25 pg/ml - 2000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

製品の概要

  • 製品名

    Human CXCL16 ELISA Kit
    CXCL16 キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Human serum 5 1.5%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Human serum 3 5.6%

  • サンプルの種類

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    2 pg/ml

  • 検出範囲

    31.25 pg/ml - 2000 pg/ml

  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 81.2 79.5% - 82.5%
    Cell culture media 108 104.3% - 114.5%
    Hep Plasma 87.1 73.5% - 101.3%
    EDTA Plasma 87.9 80.8% - 91.8%
    Cit plasma 87 85.9% - 87.8%

  • 全工程の試験時間

    1h 30m

  • ステップ

    One step assay

  • 種交差性

    交差種: Human

  • 製品の概要

    Human CXCL16 ELISA Kit (ab187397) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL16 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CXCL16 with 2 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • 特記事項

    CXCL16 is a member of the chemokine superfamily. CXCL16 may actsas a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis. CXCL16 induces a strong chemotactic response. CXCL16 may have a role in leukocytes migration. CXCL16 induces calcium mobilization.

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 試験プラットフォーム

    Microplate

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab187397の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Sandwich ELISA
Use at an assay dependent concentration.
特記事項
Sandwich ELISA
Use at an assay dependent concentration.

画像

  • Other - Human CXCL16 ELISA Kit (ab187397)
    Other - Human CXCL16 ELISA Kit (ab187397)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of CXCL16 standard curve.
    Example of CXCL16 standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Titration of Human serum and plasma within the working range of the assay.
    Titration of Human serum and plasma within the working range of the assay.

    Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Titration of HepG2 cell culture supernatant within the working range of the assay.
    Titration of HepG2 cell culture supernatant within the working range of the assay.

    Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • CXCL16 concentrations in 10 individual Human serum donors.
    CXCL16 concentrations in 10 individual Human serum donors.

    8 X diluted serum samples from 10 apparently healthy male donors were measured using this kit. Interpolated data values corrected for sample dilution are graphed in ng of CXCL16 per mL of serum (mean +/- SD, n=3). The mean of CXCL16 concentration of these ten individual serum samples was determined to be 3.26 ng /mL with a range of 2.58 – 4.08 ng /mL.

参考文献 (0)

ab187397 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab187397 は論文での使用が確認できていません。

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