AB230940

Human CSF-1-R ELISA Kit

Recombinant
SimpleStep
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Human CSF-1-R ELISA Kit is a single-wash 90-min SimpleStep ELISA used to quantify Human CSF-1-R with a sensitivity of 20.1 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab327070

別名を表示する

CD115, FMS, CSF1R, Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, Proto-oncogene c-Fms, CSF-1-R, CSF-1R, M-CSF-R

6 Images

ELISA - Human CSF-1-R ELISA Kit (AB230940)

ELISA

Supplier Data

ELISA - Human CSF-1-R ELISA Kit (AB230940)

sELISA

Supplier Data

Sandwich ELISA - Human CSF-1-R ELISA Kit (AB230940)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSF-1-R concentration was determined to be 141 ng/mL with a range of 81 – 240 ng/mL.

sELISA

Lab

Sandwich ELISA - Human CSF-1-R ELISA Kit (AB230940)

Interpolated concentrations of native CSF-1-R in human serum and plasma samples. The concentrations of CSF-1-R were measured in duplicates, interpolated from the CSF-1-R standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 400, plasma (heparin) 1 : 200, plasma (citrate) 1 : 400 and plasma (EDTA) 1 : 400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSF-1-R concentration was determined to be 163 ng/mL in neat serum, 113 ng/mL in neat plasma (heparin), 144 ng/mL in neat plasma (citrate), and 149 ng/mL in neat plasma (EDTA).

sELISA

Lab

Sandwich ELISA - Human CSF-1-R ELISA Kit (AB230940)

Interpolated concentrations of native CSF-1-R in human THP-1 cell extract, kidney extract, and liver extract. The concentrations of CSF-1-R were measured in duplicate and interpolated from the CSF-1-R standard curve and corrected for sample dilution. Undiluted samples are as follows : THP-1 cell extract 500 µg/mL, kidney extract 100 µg/mL, and liver extract 500 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CSF-1-R concentration in the undiluted samples was determined to be 2,100 pg/mL in THP-1 cell extract, 489 pg/mL in kidney extract, and 733 pg/mL in liver extract.

sELISA

Lab

Sandwich ELISA - Human CSF-1-R ELISA Kit (AB230940)

Example of human CSF-1-R standard curve in Sample Diluent NS with Enhancer prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human CSF-1-R ELISA Kit (AB230940)

Example of human CSF-1-R standard curve in Sample Diluent NS prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 20.1 pg/mL

検出範囲

78.1 - 5000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human CSF-1-R ELISA Kit ab230940 is a rapid single-wash 90-min Sandwich ELISA to measure Human CSF-1-R in cell culture extracts, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 20.1 pg/mL.

How the assay works

Human CSF-1-R SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CSF-1-R ELISA Kit ab230940 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CSF-1-R antibody [EPR20754-46] - BSA and Azide free (Capture) ab244979
- Anti-CSF-1-R antibody [EPR20754-103] - BSA and Azide free (Detector) ab244979

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "Human Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" }, { "reproducibilityType": "Intra", "sample": "Human Serum", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" } ]

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回収率

[ { "sample": "Serum", "range": "104 - 107 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "106 - 111 %", "average": "= 108" }, { "sample": "Cell culture extracts", "range": "101 - 103 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "105 - 111 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "107 - 113 %", "average": "= 110" }, { "sample": "Liver Extract", "range": "106 - 110 %", "average": "= 108" }, { "sample": "Kidney Extract", "range": "101 - 102 %", "average": "= 101" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (PubMed : 20504948, PubMed : 30982609). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (PubMed : 30982608).

See full target information CSF1R

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 22:26 PubMed39891235

2025

Rescue of in vitro models of CSF1R-related adult-onset leukodystrophy by iluzanebart: mechanisms and therapeutic implications of TREM2 agonism.

Applications

Unspecified application

Species

Unspecified reactive species

Kelley C Larson,Frederick W Gergits,Abigail J Renoux,Elizabeth J Weisman,Borislav Dejanovic,Liyue Huang,Bhaumik Pandya,Donald G McLaren,Berkley A Lynch,Richard Fisher,Evan Thackaberry,David Gray,Francois Gaudreault,Christian Mirescu

View all publications

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websiteProtocolBooklet

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Human CSF-1-R ELISA Kit