AB213768

Human CRISP3 ELISA Kit

Name: Human CRISP3 ELISA Kit (ab213768) | Abcam
Brand: Abcam Limited
SKU: AB213768
Price: 109000 JPY
Availability: InStock

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(1 Publication)

Human CRISP3 ELISA Kit is a Sandwich ELISA for the measurement of Human CRISP3 in Human in Cell Culture Media, Biofluids samples.

別名を表示する

Cysteine-rich secretory protein 3, CRISP-3, Specific granule protein of 28 kDa, SGP28, CRISP3

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Sandwich ELISA - Human CRISP3 ELISA Kit (AB213768)

sELISA

Supplier Data

Sandwich ELISA - Human CRISP3 ELISA Kit (AB213768)

Human CRISP3 ELISA Kit (ab213768) Standard Curve.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture supernatant, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine

交差種

Human

アッセイタイプ

Sandwich

検出感度

< 10 pg/mL

検出範囲

62.5 - 4000 pg/mL

アッセイ時間

3h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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製品の詳細

The Human CRISP3 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213768) is designed for the quantitative measurement of Human CRISP3 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine.

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for CRISP3 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: N21-Y245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CRISP3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CRISP3 amount of sample captured in plate.

Cysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. CRISP3 expression is found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. It is a potential biomarker for prostate cancer. CRISP3 gene can be defined as an early-transcribed gene with a putative role in the pathophysiology of sjogren's syndrome. CRISP3 is present in exocrine secretions and in secretory granules of neutrophilic granulocytes and is believed to play a role in innate immunity.

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精度

[ { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "408 pg/mL", "standardDeviation": "26.11", "coefficientOfVariability": "= 6.4" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "1764 pg/mL", "standardDeviation": "95.26", "coefficientOfVariability": "= 5.4" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "110 pg/mL", "standardDeviation": "4.51", "coefficientOfVariability": "= 4.1" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "119 pg/mL", "standardDeviation": "5.59", "coefficientOfVariability": "= 4.7" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "383 pg/mL", "standardDeviation": "23.75", "coefficientOfVariability": "= 6.2" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "1477 pg/mL", "standardDeviation": "66.47", "coefficientOfVariability": "= 4.5" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CRISP3 also known as Cysteine-Rich Secretory Protein 3 is a protein key in various physiological processes. It typically weighs about 28 kDa. Found in a range of tissues but most prominently in the salivary glands pancreas and prostate. Researchers also detect CRISP3 in neutrophils. Its secretion to the extracellular environment after synthesis helps facilitate its functional roles. Scientists use ELISA assays to quantify its expression.

Biological function summary

CRISP3 participates in host defense mechanisms and inflammation. It is a part of antimicrobial complexes that safeguard the body against microbial invasion. This protein can bind to other molecules and disrupt microbial membranes. It is believed to be involved in the innate immune response. CRISP3 also appears in granules of neutrophils where it functions during pathogen encounters.

Pathways

CRISP3 interacts in immune response pathways that help buffer the body against pathogenic challenges. It aligns with the immune system's defense activities and might impact the signaling and regulatory pathways of inflammation. CRISP3's interactions with other proteins like defensins enhance its role in these immune pathways.

CRISP3 shows potential involvement in prostate cancer and Sjögren's syndrome. Its overexpression associates with prostate cancer progression and alterations in its levels might affect cancer-related proteins like PSA (prostate-specific antigen). In the context of Sjögren's syndrome CRISP3's expression changes may link to saliva secretion dysregulation impacting mucosal defense and inflammatory proteins involved in the disorder.

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製品プロトコール

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ターゲットの情報

See full target information Cysteine-rich secretory protein 3

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Bioscience reports 39: PubMed31300526

2019

Investigation of serum proteome homeostasis during radiation therapy by a quantitative proteomics approach.

Applications

Unspecified application

Species

Unspecified reactive species

Amira Ouerhani,Giovanni Chiappetta,Oussema Souiai,Halima Mahjoubi,Joelle Vinh

View all publications

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Human CRISP3 ELISA Kit