Human Coxsackie Adenovirus Receptor/hCAR ELISA Kit (ab300308)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.45 pg/ml
- Range: 25 pg/ml - 1600 pg/ml
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Coxsackie Adenovirus Receptor/hCAR ELISA Kit
Coxsackie Adenovirus Receptor/hCAR キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Extract 8 2.6% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Extract 3 4.7% -
サンプルの種類
Cell Lysate -
アッセイタイプ
Sandwich (quantitative) -
検出感度
2.45 pg/ml -
検出範囲
25 pg/ml - 1600 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell Lysate 92 86% - 96% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human Coxsackie Adenovirus Receptor/hCAR SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Coxsackie Adenovirus Receptor/hCAR protein in human cell extract. Quantitate Human Coxsackie Adenovirus Receptor/hCAR with 2.45 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 96 tests 10X Human Coxsackie Adenovirus Receptor/hCAR Capture Antibody 1 x 600µl 10X Human Coxsackie Adenovirus Receptor/hCAR Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human Coxsackie Adenovirus Receptor/hCAR Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN. -
組織特異性
Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy. -
配列類似性
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
ドメイン
The Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML.
The PDZ-binding motif mediates interaction with MPDZ and BAIAP1. -
翻訳後修飾
N-glycosylated.
Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane. -
細胞内局在
Secreted and Cell membrane. Cell junction > tight junction. Cell junction > adherens junction. Basolateral cell membrane. In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface. - Information by UniProt
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別名
- 46 kD coxsackievirus and adenovirus receptor CAR protein
- 46 kD coxsackievirus and adenovirus receptor protein
- CAR
see all -
参照データベース
- Entrez Gene: 1525 Human
- Omim: 602621 Human
- SwissProt: P78310 Human
- Unigene: 634837 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300308の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Example of human Coxsackie Adenovirus Receptor/hCAR standard curve in 1X Cell Extraction Buffer PTR.
Example of human Coxsackie Adenovirus Receptor/hCAR standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of human Coxsackie Adenovirus Receptor/hCAR in Hela cell extract.Interpolated concentration of native Coxsackie Adenovirus Receptor/hCAR was measured in duplicate at different sample concentrations. Undiluted samples are 100 µg/mL Hela cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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Human Coxsackie Adenovirus Receptor/hCAR ELISA Kit (ab300308) -
Human Coxsackie Adenovirus Receptor/hCAR ELISA Kit (ab300308)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab300308 は論文での使用が確認できていません。