Human Complement C4 ELISA Kit (ab108824)
Key features and details
- Sensitivity: 0.53 µg/ml
- Range: 0.313 µg/ml - 5 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
製品の概要
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製品名
Human Complement C4 ELISA Kit
C4 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 5.1% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 10.1% -
サンプルの種類
Serum, Plasma -
アッセイタイプ
Competitive -
検出感度
= 0.53 µg/ml -
検出範囲
0.313 µg/ml - 5 µg/ml -
添加回収試験
94 %
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全工程の試験時間
3h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Complement C4 Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab108834) is designed for the quantitative measurement of Complement C4 levels in plasma and serum.
A Complement C4 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Complement C4 is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Complement C4 captured in plate.
Reactivity with C4 cleavage products (C4a, C4b, C4d) has not been determined.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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アプリケーション
適用あり: Competitive ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml 2X Biotinylated Human Complement C4 (Lyophilized) 1 vial Chromogen Substrate 1 x 7ml Complement C4 Microplate (12 x 8 well strips) 1 unit Complement C4 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
- Information by UniProt
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別名
- Acidic C4
- Acidic complement C4
- Basic C4
see all -
参照データベース
- Entrez Gene: 720 Human
- Entrez Gene: 721 Human
- Entrez Gene: 100293534 Human
- Entrez Gene: 721 Human
- Omim: 120810 Human
- Omim: 120820 Human
- SwissProt: P0C0L4 Human
- SwissProt: P0C0L5 Human
see all
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108824の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. |
画像
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ab108824 Complement C4 Human ELISA Kit
Complement C4 measured in biological fluids showing quantity (microgram) per mL of tested sample
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ab108824 Complement C4 Human ELISA KitStandard curve : mean of duplicates (+/-SD)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab108824 は 9 報の論文で使用されています。
- Severance EG et al. Prospects and Pitfalls of Plasma Complement C4 in Schizophrenia: Building a Better Biomarker. Dev Neurosci 45:349-360 (2023). PubMed: 37734326
- Gallego JA et al. Complement component C4 levels in the cerebrospinal fluid and plasma of patients with schizophrenia. Neuropsychopharmacology 46:1140-1144 (2021). PubMed: 32961544
- Martin M et al. Plasma C4d Correlates With C4d Deposition in Kidneys and With Treatment Response in Lupus Nephritis Patients. Front Immunol 11:582737 (2020). PubMed: 33133102
- Salazar-Camarena DC et al. Analysis of the receptor BCMA as a biomarker in systemic lupus erythematosus patients. Sci Rep 10:6236 (2020). PubMed: 32277232
- Cleary SJ et al. Complement activation on endothelium initiates antibody-mediated acute lung injury. J Clin Invest 130:5909-5923 (2020). PubMed: 32730229
- Qu S et al. MicroRNA-194 reduces inflammatory response and human dermal microvascular endothelial cells permeability through suppression of TGF-ß/SMAD pathway by inhibiting THBS1 in chronic idiopathic urticaria. J Cell Biochem 121:111-124 (2020). PubMed: 31190349
- Walss-Bass C et al. X-Aptamer Technology Identifies C4A and ApoB in Blood as Potential Markers for Schizophrenia. Mol Neuropsychiatry 5:52-59 (2019). PubMed: 31019918
- Liu Y et al. Toll-like receptor-9 is involved in the development of B cell stimulating factor-induced systemic lupus erythematosus. Exp Ther Med 15:585-591 (2018). PubMed: 29387207
- Owsley C et al. Associations between abnormal rod-mediated dark adaptation and health and functioning in older adults with normal macular health. Invest Ophthalmol Vis Sci 55:4776-89 (2014). ELISA ; Human . PubMed: 24854857