AB324923

Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit

SimpleStep
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Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Complement C4 Binding Protein Beta (C4BPB) with a sensitivity of 54.47pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders and 384-well for higher throughput.

別名を表示する

C4b-binding protein beta chain, C4BPB

6 Images

Sandwich ELISA - Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit (AB324923)

sELISA

Lab

Sandwich ELISA - Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit (AB324923)

Interpolated concentration of native Complement C4 Binding Protein Beta was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 2.5% human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Sample Diluent SSW.

sELISA

Lab

Sandwich ELISA - Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit (AB324923)

Example of human Complement C4 Binding Protein Beta standard curve in 1X Sample Diluent SSW. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit (AB324923)

Interpolated concentration of native Complement C4 Binding Protein Beta was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human serum 5%, human plasma (citrate) 5%, and human plasma (heparin) 5%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Sample Diluent SSW.

sELISA

Lab

Sandwich ELISA - Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit (AB324923)

Example of human Complement C4 Binding Protein Beta standard curve in 96-well vs. 384-well plate in 1X Sample Diluent SSW. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit (AB324923)

Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Complement C4 Binding Protein Beta concentration was determined to be 30.6 ng/mL with a range of 17.8 – 42.8 ng/mL.

sELISA

Lab

Sandwich ELISA - Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit (AB324923)

Interpolated concentration of native Complement C4 Binding Protein Beta was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human plasma (EDTA) 2.5%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Sample Diluent SSW.

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 54.47 pg/mL

検出範囲

117.188 - 7500 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit (ab324923) is a rapid single-wash sandwich 90-min ELISA to measure Human Complement C4 Binding Protein Beta (C4BPB) in Serum, Plasma – Citrate, Plasma – EDTA, Plasma – Heparin, Cell Culture Media. This SimpleStep sensitivity is 54.47 pg/ml.

How the assay works

Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit (ab324923) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Complement C4 Binding Protein Beta (C4BPB) (ab324923) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit only requires 12.5µL sample.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.3" } ]

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回収率

[ { "sample": "Serum", "range": "85 - 97 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "91 - 97 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "87 - 94 %", "average": "= 90" }, { "sample": "Heparin Plasma", "range": "81 - 88 %", "average": "= 84" }, { "sample": "Cell culture media", "range": "90 - 100 %", "average": "= 95" } ]

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製品内容

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab324923-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB324923-CMP12" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB324923-CMP10" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB324923-CMP09" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB324923-CMP08" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB324923-CMP06" }, { "size":"2 x 50 mL", "name":"Cell Extraction Buffer SSW", "number":"AB324923-CMP05" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB324923-CMP04" }, { "size":"20 x 1 Vial", "name":"Human Complement C4 Binding Protein Beta (C4BPB) Lyophilized Recombinant Protein", "number":"AB324923-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human Complement C4 Binding Protein Beta (C4BPB) Detector Antibody", "number":"AB324923-CMP02" }, { "size":"10 x 600 µL", "name":"10X Human Complement C4 Binding Protein Beta (C4BPB) Capture Antibody", "number":"AB324923-CMP01" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab324923-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB324923-CMP12" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB324923-CMP09" }, { "size":"2 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB324923-CMP06" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB324923-CMP11" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB324923-CMP08" }, { "size":"1 x 50 mL", "name":"Cell Extraction Buffer SSW", "number":"AB324923-CMP05" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB324923-CMP04" }, { "size":"2 x 1 Vial", "name":"Human Complement C4 Binding Protein Beta (C4BPB) Lyophilized Recombinant Protein", "number":"AB324923-CMP03" }, { "size":"1 x 600 µL", "name":"10X Human Complement C4 Binding Protein Beta (C4BPB) Detector Antibody", "number":"AB324923-CMP02" }, { "size":"1 x 600 µL", "name":"10X Human Complement C4 Binding Protein Beta (C4BPB) Capture Antibody", "number":"AB324923-CMP01" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab324923-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB324923-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB324923-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB324923-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB324923-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB324923-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB324923-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"Cell Extraction Buffer SSW", "number":"AB324923-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB324923-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Complement C4 Binding Protein Beta (C4BPB) Lyophilized Recombinant Protein", "number":"AB324923-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Complement C4 Binding Protein Beta (C4BPB) Detector Antibody", "number":"AB324923-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Complement C4 Binding Protein Beta (C4BPB) Capture Antibody", "number":"AB324923-CMP01", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. It also interacts with anticoagulant protein S and with serum amyloid P component. The beta chain binds protein S.

See full target information C4BPB

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websiteProtocolBooklet

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Abcam product promise

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Human Complement C4 Binding Protein Beta (C4BPB) ELISA Kit