AB178633

Human Chorionic Gonadotropin beta ELISA Kit

Name: Human Chorionic Gonadotropin beta ELISA Kit (ab178633) | Abcam
Brand: Abcam Limited
SKU: AB178633
Price: 104000 JPY
Availability: InStock

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(2 Publications)

Human Chorionic Gonadotropin beta ELISA Kit is an indirect ELISA for the qualitative detection of class antibodies against Chorionic Gonadotropin beta ELISA Kit in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation

別名を表示する

Choriogonadotropin subunit beta 7, CGB7

Key facts

検出方法

Colorimetric

サンプルタイプ

Citrate plasma, Heparin Plasma, Serum

交差種

Human

アッセイタイプ

Sandwich (quantitative)

検出感度

> 0.09 mU/mL

検出範囲

1 - 400 mU/mL

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Abcam's Chorionic Gonadotropin beta in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Chorionic Gonadotropin beta in Human serum and plasma.

A 96-well plate has been precoated with anti-Chorionic Gonadotropin beta IgG antibodies. Samples, standards and the Chorionic Gonadotropin beta-HRP conjugate are added to the wells, where Chorionic Gonadotropin beta in the sample and standards binds to the precoated antibody and added Chorionic Gonadotropin beta-HRP conjugate binds to this antibody-Chorionic Gonadotropin beta complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Chorionic Gonadotropin beta in the sample and the intensity is measured at 450 nm.

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精度

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 48, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 7.6" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 8.8" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab178633-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 mL", "name":"Chorionic Gonadotropin beta Standard 0 - 0 mU/mL", "number":"AB178633-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover Foil", "number":"AB178633-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB178633-CMP15", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Chorionic Gonadotropin beta Coated Microplate (12 x 8 wells)", "number":"AB178633-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"Chorionic Gonadotropin beta Standard 4 - 100 mU/mL", "number":"AB178633-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB178633-CMP13", "productcode":"" }, { "size":"1 x 1 mL", "name":"Chorionic Gonadotropin beta Standard 5 - 400 mU/mL", "number":"AB178633-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Incubation Buffer", "number":"AB178633-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"50X Washing Solution", "number":"AB178633-CMP01", "productcode":"" }, { "size":"1 x 1 mL", "name":"10X Anti-Chorionic Gonadotropin beta HRP Conjugate", "number":"AB178633-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"Chorionic Gonadotropin beta Standard 1 - 1 mU/mL", "number":"AB178633-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"Chorionic Gonadotropin beta Standard 3 - 20 mU/mL", "number":"AB178633-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"Chorionic Gonadotrophin beta Control", "number":"AB178633-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"Chorionic Gonadotropin beta Standard 2 - 5 mU/mL", "number":"AB178633-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB178633-CMP14", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Chorionic Gonadotropin beta also known as β-hCG is a major subunit of the human chorionic gonadotropin hormone. It is a glycoprotein with a molecular mass of approximately 22 kD. β-hCG predominantly expresses in placental syncytiotrophoblast cells and appears in the blood and urine. This hormone plays an essential role in reproductive processes and researchers often measure it using β-hCG ELISA kits.

Biological function summary

The beta subunit promotes pregnancy maintenance by supporting corpus luteum function and embryo development. β-hCG exists as part of the larger hCG hormone complex which includes an alpha subunit that it shares with other glycoproteins like luteinizing hormone (LH) and follicle-stimulating hormone (FSH). Its binding to LH/CG receptors in the ovary makes it pivotal for progesterone secretion in early pregnancy.

Pathways

Chorionic Gonadotropin beta engages in reproductive hormone pathways significantly impacting the gonadotropin-releasing hormone (GnRH) signaling pathway. It relates to LH directly and its actions are indispensable in stimulating ovarian androgen secretion. These pathways further link it to progesterone production emphasizing its importance in fertility and pregnancy.

Chorionic Gonadotropin beta has notable connections with gestational trophoblastic diseases including molar pregnancy and choriocarcinoma. Elevated levels of β-hCG also indicate potential testicular cancers in males. Researchers recognize its association with abnormal cellular growth and its measurements are useful in monitoring these malignancies. Furthermore its interaction with LH and FSH places it within hormonal imbalances that can result in fertility issues.

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製品プロトコール

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ターゲットの情報

Beta subunit of the human chorionic gonadotropin (hCG). hCG is a complex glycoprotein composed of two glycosylated subunits alpha and beta which are non-covalently associated. The alpha subunit is identical to those in the pituitary gonadotropin hormones (LH, FSH and TSH). The beta subunits are distinct in each of the hormones and confer receptor and biological specificity. Has an essential role for pregnancy and maternal adaptation. Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.

See full target information Choriogonadotropin subunit beta 7

追加のターゲット

Choriogonadotropin subunit beta 7

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:2777-2790 PubMed35038951

2022

ZBTB24 (Zinc Finger and BTB Domain Containing 24) prevents recurrent spontaneous abortion by promoting trophoblast proliferation, differentiation and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Haibo Ruan,Zhenzhen Dai,Jinyu Yan,Xiaoxi Long,Yi Chen,Youlin Yang,Qian Yang,Jun Zhu,Meiyun Zheng,Xiahui Zhang

Nature 601:600-605 PubMed34856602

2021

Human blastoids model blastocyst development and implantation.

Applications

Unspecified application

Species

Unspecified reactive species

Harunobu Kagawa,Alok Javali,Heidar Heidari Khoei,Theresa Maria Sommer,Giovanni Sestini,Maria Novatchkova,Yvonne Scholte Op Reimer,Gaël Castel,Alexandre Bruneau,Nina Maenhoudt,Jenna Lammers,Sophie Loubersac,Thomas Freour,Hugo Vankelecom,Laurent David,Nicolas Rivron

View all publications

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websiteProtocolBooklet

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Abcam product promise

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Human Chorionic Gonadotropin beta ELISA Kit