Human Anti-Chlamydia pneumoniae IgA ELISA Kit (ab108719)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
製品の概要
-
製品名
Human Anti-Chlamydia pneumoniae IgA ELISA Kit -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Pos. Serum 20 4.9% Pos. Serum 24 8.3% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Pos. Serum 12 9.7% Pos. Serum 12 14% -
サンプルの種類
Serum, Hep Plasma, Cit plasma -
アッセイタイプ
Indirect -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s anti-Chlamydia pneumoniae IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Chlamydia pneumoniae in Human serum and plasma.
A 96-well plate has been precoated with Chlamydia pneumoniae antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Chlamydia pneumoniae-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Chlamydia pneumoniae IgA sample captured in plate.
-
アプリケーション
適用あり: ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Chlamydia pneumoniae (IgA) Coated Microplate (12 x 8 wells) 1 unit Chlamydia pneumoniae anti-IgA HRP Conjugate Colored violet, black cap 1 x 20ml Chlamydia pneumoniae IgA Cut Off Control Colored yellow, green cap 1 x 3ml Chlamydia pneumoniae IgA Negative Control Colored yellow, blue cap 1 x 2ml Chlamydia pneumoniae IgA Positive Control Colored yellow, red cap. 1 x 2ml IgA Sample Diluent Colored yellow; white cap 1 x 100ml Stop Solution red cap 1 x 15ml TMB Substrate Solution Yellow cap 1 x 15ml -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108719の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. |
画像
-
Serologic ELISA assay principleSpecific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab108719 は論文での使用が確認できていません。