AB227903

Human CDKN2A/p16INK4a ELISA Kit

Recombinant
SimpleStep
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(2 Publications)

Human CDKN2A/p16INK4a ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CDKN2A/p16INK4a with a sensitivity of 1.3 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

CDKN2, MTS1, CDKN2A, Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A, Multiple tumor suppressor 1, p16-INK4a, CDK4I, MTS-1, p16-INK4, p16INK4A

4 Images

ELISA - Human CDKN2A/p16INK4a ELISA Kit (AB227903)

ELISA

Supplier Data

ELISA - Human CDKN2A/p16INK4a ELISA Kit (AB227903)

sELISA

Supplier Data

Sandwich ELISA - Human CDKN2A/p16INK4a ELISA Kit (AB227903)

Example of Human CDKN2A standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human CDKN2A/p16INK4a ELISA Kit (AB227903)

Interpolated concentrations of native CDKN2A in Human HeLa extract based on a 500 μg/mL extract load.

The concentrations of CDKN2A/p16INK4a were measured in duplicate and interpolated from the CDKN2A/p16INK4a standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CDKN2A/p16INK4a concentration was determined to be 73 ng/mL in HeLa cell extract.

sELISA

Supplier Data

Sandwich ELISA - Human CDKN2A/p16INK4a ELISA Kit (AB227903)

Interpolated concentrations of spiked CDKN2A in Human Fetal Skeletal Muscle tissue homogenate extract based on a 250 μg/mL extract load.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture extracts, Tissue Extracts

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 1.3 ng/mL

検出範囲

2.3 - 150 ng/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human CDKN2A/p16INK4a ELISA Kit ab227903 is a rapid single-wash 90-min Sandwich ELISA to measure Human CDKN2A/p16INK4a in cell culture extracts, tissue extracts. This SimpleStep sensitivity is 1.3 ng/mL.

How the assay works

Human CDKN2A/p16INK4a SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CDKN2A/p16INK4a ELISA Kit ab227903 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CDKN2A/p16INK4a antibody [EPR20419-75] - BSA and Azide free (Capture) ab244961
- Anti-CDKN2A/p16INK4a antibody [EPR20419-262] - BSA and Azide free (Detector) ab244961

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "HEK-293T Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" }, { "reproducibilityType": "Intra", "sample": "HEK-293T Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]

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回収率

[ { "sample": "Cell culture extracts", "range": "110 - 125 %", "average": "= 116" }, { "sample": "Tissue Extracts", "range": "92 - 96 %", "average": "= 95" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

The protein expressed by the gene CDKN2A acts as a negative regulator of normal cell proliferation by strongly interacting with CDK4 and CDK6. This interaction inhibits the ability of CDK4 and CDK6 to interact with cyclins D and to phosphorylate the retinoblastoma protein. This supplementary information is collated from multiple sources and compiled automatically.

See full target information CDKN2A

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Infectious diseases (London, England) 54:918-923 PubMed35984738

2022

Markers of cellular senescence is associated with persistent pulmonary pathology after COVID-19 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Tove Lekva,Thor Ueland,Bente Halvorsen,Sarah Louise Murphy,Anne Ma Dyrhol-Riise,Anders Tveita,Ane-Kristine Finbråten,Alexander Mathiessen,Karl Erik Müller,Trond Mogens Aaløkken,Ole Henning Skjønsberg,Tøri Vigeland Lerum,Pål Aukrust,Tuva Børresdatter Dahl

American journal of reproductive immunology (New Y :e13394 PubMed33501727

2021

Triaging abnormal cervical cancer screening tests using p16INK4a detection by ELISA on fresh cervical samples.

Applications

Unspecified application

Species

Unspecified reactive species

Shuk On Annie Leung,Sarah Feldman,Rajeshwari Kalyanaraman,Vignesh Shanmugam,Michael J Worley,Ross S Berkowitz,Neil S Horowitz,Colleen M Feltmate,Michael G Muto,Larissa J Lee,Martin T King,Jon I Einarsson,Mobolaji O Ajao,Kevin M Elias

View all publications

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websiteProtocolBooklet

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Human CDKN2A/p16INK4a ELISA Kit