Human CD62L ELISA Kit (Selectin L) (ab45917)
Key features and details
- Sensitivity: 1 ng/ml
- Range: 1.9 ng/ml - 60 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human CD62L ELISA Kit (Selectin L)
CD62L キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Sample A 15 28ng/ml 1.3 4.6% Sample B 19 9ng/ml 0.5 5.5% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 1 ng/ml -
検出範囲
1.9 ng/ml - 60 ng/ml -
全工程の試験時間
1h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s CD62L (Selectin L) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD62L in Human serum, plasma, buffered solutions and cell culture medium.
A monoclonal antibody specific for CD62L has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD62L concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD62L are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD62L present in the samples.
This kit will recognize both endogenous and recombinant Human CD62L.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml Biotinylated anti-CD62L antibody Red 1 x 0.4ml 2 x 0.4ml CD62L Control Silver 2 vials 4 vials CD62L Microplate (12 x 8 well strips) 1 unit 2 units CD62L Standard Yellow 2 vials 4 vials Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 11ml 1 x 23ml Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究分野
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関連性
CD62L is a cell surface component that is a member of a family of adhesion/homing receptors. These play important roles in leukocyte-endothelial cell interactions. CD62L is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins. -
細胞内局在
Cell Membrane; single-pass type I membrane protein. -
別名
- A.11
- AI528707
- CD62 antigen ligand
see all -
参照データベース
- Entrez Gene: 6402 Human
- Omim: 153240 Human
- SwissProt: P14151 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab45917の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Typical Standard Curve
Representative Standard Curve using ab45917
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab45917 は 1 報の論文で使用されています。
- Chen R et al. Differentially expressed RNA from public microarray data identifies serum protein biomarkers for cross-organ transplant rejection and other conditions. PLoS Comput Biol 6: (2010). ELISA ; Human . PubMed: 20885780