Human CD44 ELISA Kit (ab45912)
Key features and details
- Sensitivity: 0.113 ng/ml
- Range: 0.31 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
-
製品名
Human CD44 ELISA Kit
CD44 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Sample A 8 9.85ng/ml 1.55 1.52% Sample B 8 10.5ng/ml 0.055 4.76% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Sample A 50 9.95ng/ml 0.307 3.08% Sample B 50 1.07ng/ml 0.105 9.34% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 0.113 ng/ml -
検出範囲
0.31 ng/ml - 10 ng/ml -
全工程の試験時間
2h 15m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s CD44 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble CD44 in Human serum, plasma, buffered solutions and cell culture medium.
A monoclonal antibody specific for CD44 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD44 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD44 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD44 present in the samples.
This kit will recognize both endogenous and recombinant Human CD44.
-
アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 2 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 200X Wash Buffer White 2 x 10ml 1 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7ml Biotinylated anti-CD44 anitibody Red 2 x 0.4ml 1 x 0.4ml CD44 Control Silver 4 vials 2 vials CD44 Microplate (12 x 8 well strips) 2 units 1 unit CD44 Standard Yellow 4 vials 2 vials Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml HRP Diluent Red 1 x 23ml 1 x 12ml Plastic Plate Covers 4 units 2 units Stop Reagent Black 2 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl -
研究分野
-
機能
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
組織特異性
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
配列類似性
Contains 1 Link domain. -
ドメイン
The lectin-like LINK domain is responsible for hyaluronan binding. -
翻訳後修飾
Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
細胞内局在
Membrane. - Information by UniProt
-
別名
- LHR
- BA-1
- CD 44
see all -
参照データベース
- Entrez Gene: 960 Human
- Omim: 107269 Human
- SwissProt: P16070 Human
- Unigene: 502328 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab45912の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
-
Dose Response Curve (Logarithmic)Representative Dose Response Curve (Logarithmic) using ab45912.
-
Sandwich ELISA - hu CD44 ELISA kit (ab45912)huCD44 measured in biofluids showing quantity (ng) per mL of tested sample
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (7)
ab45912 は 7 報の論文で使用されています。
- Shao Y et al. CD44/ERM/F-actin complex mediates targeted nuclear degranulation and excessive neutrophil extracellular trap formation during sepsis. J Cell Mol Med 26:2089-2103 (2022). PubMed: 35146909
- Cenariu D et al. Zeaxanthin-Rich Extract from Superfood Lycium barbarum Selectively Modulates the Cellular Adhesion and MAPK Signaling in Melanoma versus Normal Skin Cells In Vitro. Molecules 26:N/A (2021). PubMed: 33440679
- Wang Y et al. [Expression of CD44 in Tumor Tissue and Serum of Small Cell Lung Cancer and Its Clinical Prognostic Significance]. Zhongguo Fei Ai Za Zhi 24:583-590 (2021). PubMed: 34187156
- Lau WM et al. CD44v8-10 is a cancer-specific marker for gastric cancer stem cells. Cancer Res 74:2630-41 (2014). PubMed: 24618343
- Simpson KL et al. A caspase-3 'death-switch' in colorectal cancer cells for induced and synchronous tumor apoptosis in vitro and in vivo facilitates the development of minimally invasive cell death biomarkers. Cell Death Dis 4:e613 (2013). ELISA ; Human . PubMed: 23640455
- Urquidi V et al. CCL18 in a Multiplex Urine-Based Assay for the Detection of Bladder Cancer. PLoS One 7:e37797 (2012). ELISA ; Human . PubMed: 22629457
- Chen R et al. Differentially expressed RNA from public microarray data identifies serum protein biomarkers for cross-organ transplant rejection and other conditions. PLoS Comput Biol 6: (2010). ELISA ; Human . PubMed: 20885780