AB234569

Human CD4 ELISA Kit

Recombinant
SimpleStep
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Human CD4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD4 with a sensitivity of 13.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

CD4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

3 Images

Sandwich ELISA - Human CD4 ELISA Kit (AB234569)

sELISA

Supplier Data

Sandwich ELISA - Human CD4 ELISA Kit (AB234569)

Representative image of Human CD4 ELISA Kit (ab234569)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human CD4 ELISA Kit Capture Antibody

- 10X Human CD4 Detector Antibody

- 10X Human CD4 ELISA Kit Lyophilised Purified Protein (2 vials)

- Human CD4 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Human CD4 ELISA Kit (AB234569)

Interpolated concentrations of native CD4 in human HL-60 Extract based on a 500 μg/mL extract load.

The concentrations of CD4 were measured in duplicate and interpolated from the CD4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD4 concentration was determined to be 8.95 ng/mL in HL-60 extract.

sELISA

Supplier Data

Sandwich ELISA - Human CD4 ELISA Kit (AB234569)

Example of human CD4 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture extracts

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 13.7 pg/mL

検出範囲

0.469 - 30 ng/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human CD4 ELISA Kit (ab234569) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD4 protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD4 with 13.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.4" }, { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.2" } ]

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回収率

[ { "sample": "Cell culture extracts", "range": "96 - 105 %", "average": "= 100" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab234569-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB234569-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB234569-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB234569-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB234569-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB234569-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB234569-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB234569-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB234569-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB234569-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD4 Capture Antibody", "number":"AB234569-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CD4 Lyophilized Recombinant Protein", "number":"AB234569-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD4 Detector Antibody", "number":"AB234569-CMP09", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed : 12089508, PubMed : 16331979, PubMed : 2214026, PubMed : 9641677). Down-regulated by HIV-1 Vpu (PubMed : 17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed : 7909607).

See full target information CD4

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 19:e0304408 PubMed38923958

2024

Immunological and molecular assessment of HIV-1 mutations for antiretroviral drug resistance in Saudi Arabia.

Applications

Unspecified application

Species

Unspecified reactive species

Mai M El-Daly,Kawther A Zaher,Eitezaz A Zaki,Leena H Bajrai,Mohammad M Alhazmi,Ahmed Abdulhaq,Esam I Azhar

View all publications

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websiteProtocolBooklet

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Abcam product promise

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Human CD4 ELISA Kit