AB277708

Human CD3 Epsilon ELISA Kit

Recombinant
SimpleStep
詳細を見る

Be the first to review this product! Submit a review

(0 Publication)

Human CD3 Epsilon ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD3 Epsilon with a sensitivity of 38.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

CD3e, T3E, CD3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain

3 Images

Sandwich ELISA - Human CD3 Epsilon ELISA Kit (AB277708)

sELISA

Supplier Data

Sandwich ELISA - Human CD3 Epsilon ELISA Kit (AB277708)

Example of human CD3 epsilon standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.

The CD3 epsilon standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human CD3 Epsilon ELISA Kit (AB277708)

The CD3 epsilon standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

The concentrations of CD3 epsilon were measured in duplicate and interpolated from the CD3 epsilon standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human CD3 Epsilon ELISA Kit (AB277708)

Example of human CD3 epsilon standard curve in Sample Diluent NS.

The CD3 epsilon standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture supernatant, Serum, Cell Lysate

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 38.1 pg/mL

検出範囲

84 - 5400 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

製品の詳細

Human CD3 Epsilon ELISA kit (ab277708) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CD3 Epsilon in human serum, cell culture supernatant, and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD3 Epsilon with 38.1 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

T-Cell Surface Glycoprotein CD3 epsilon chain (CD3e), also known as T-cell surface antigen T3/Leu-4 epsilon is a type I membrane protein encoded by the CD3E gene. CD3e forms the T-cell receptor (TCR)-CD3 complex on T-lymphocyte cell surfaces by forming two heterodimers CD3E/CD3D (delta) and CD3E/CD3G (gamma). This complex plays a role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3e contains an immunoreceptor tyrosine-based activation motif (ITAM) in the cytoplasmic domain. When engaged by TCR, these motifs are phosphorylated by Src protein family kinases LCK and FYN, which activate downstream signaling pathways.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

精度

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.3" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" } ]

style

left-column

回収率

[ { "sample": "Cell culture supernatant", "range": "83 - 101 %", "average": "= 89" }, { "sample": "Serum", "range": "114 - 116 %", "average": "= 115" }, { "sample": "Cell Lysate", "range": "105 - 120 %", "average": "= 110" } ]

style

left-column

製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277708-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB277708-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB277708-CMP14", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB277708-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB277708-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB277708-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB277708-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB277708-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB277708-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB277708-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CD3 epsilon Lyophilized Recombinant Protein", "number":"AB277708-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD3 epsilon Capture Antibody", "number":"AB277708-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD3 epsilon Detector Antibody", "number":"AB277708-CMP02", "productcode":"" } ] } } }

style

left-column

出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

style

left-column

style

left-column

製品プロトコール

style

left-column

ターゲットの情報

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response (PubMed : 15294938, PubMed : 15546002, PubMed : 2470098, PubMed : 40592325, PubMed : 8490660). When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z (PubMed : 2470098, PubMed : 40592325). All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain (PubMed : 2470098, PubMed : 40592325). Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098, PubMed : 40592325). CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (By similarity). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (By similarity). Also participates in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed : 10384095, PubMed : 26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1 (PubMed : 38614099). Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed : 12110186, PubMed : 38614099).

See full target information CD3E

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CD3 Epsilon ELISA Kit