Human CD262 ELISA Kit (TRAIL R2) (ab113340)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 2.46 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human CD262 ELISA Kit (TRAIL R2) -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 2 pg/ml -
検出範囲
2.46 pg/ml - 600 pg/ml -
添加回収試験
90 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 97.22 88% - 107% Plasma 78.79 68% - 97% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s CD262 (TRAIL R2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD262 in plasma and cell culture supernatants. (Human CD262 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human CD262 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD262 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD262 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD262 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human CD262 in normal serum with ab113340, only in serum spiked with Human CD262.
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特記事項
Optimization may be required with urine samples.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human CD262 2 vials CD262 Microplate (12 x 8 wells) 1 unit Recombinant Human CD262 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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参照データベース
- SwissProt: O14763 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113340の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Representative Standard Curve
Representative Standard Curve using ab113340
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Representative Standard CurveRepresentative Standard Curve using ab113340
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab113340 は論文での使用が確認できていません。