Human CD244 ELISA Kit (2B4) (ab213758)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human CD244 ELISA Kit (2B4)
2B4 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% 1 16 0.45ng/ml 0.022 4.9% 2 16 1.2ng/ml 0.076 6.3% 3 16 3.2ng/ml 0.186 5.8% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% 1 24 0.43ng/ml 0.027 6.2% 2 24 1.3ng/ml 0.098 7.5% 3 24 2.9ng/ml 0.194 6.7% -
サンプルの種類
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 10 pg/ml -
検出範囲
15.6 pg/ml - 1000 pg/ml -
全工程の試験時間
3h 30m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
The Human CD244 Enzyme-Linked Immunosorbent Assay (ELISA) kit (2B4) (ab213758) is designed for the quantitative measurement of Human CD244 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for CD244/2B4 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: C22-R221) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD244/2B4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CD244/2B4 amount of sample captured in plate.
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特記事項
CD244 (Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Members of the CD2 family adhere to each other instead, the cell surface glycoprotein 2B4 is related to CD2 and is implicated in the regulation of NK and T-cell function. The 2B4 gene was mapped to 1q22 on gene map, where SLAM, CD48, CD84 and LY9 are also located. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, It was found that CD48 binds to 2B4. BIAcore surface plasmon resonance analysis showed that the affinity of CD48 for 2B4 is approximately 10-fold higher than that shown for CD48 and CD2.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human CD244 coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Human CD244 antibody 1 x 100µl Lyophilized recombinant Human CD244 standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
研究分野
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機能
Modulate other receptor-ligand interactions to enhance leukocyte activation. -
組織特異性
Expressed in spleen, PBL, followed by lung, liver, testis and small intestine. Expressed not only in NK cells, but also on monocytes and basophils. -
配列類似性
Contains 2 Ig-like (immunoglobulin-like) domains. -
細胞内局在
Membrane. - Information by UniProt
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別名
- 2B4
- C9.1
- CD244
see all -
参照データベース
- Entrez Gene: 51744 Human
- Omim: 605554 Human
- SwissProt: Q9BZW8 Human
- Unigene: 157872 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab213758の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Human CD244 ELISA Kit (2B4) (ab213758) Standard Curve.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab213758 は論文での使用が確認できていません。