AB100507

Human CD105 ELISA Kit (Endoglin)

Name: Human CD105 ELISA Kit (Endoglin) (ab100507) | Abcam
Brand: Abcam Limited
SKU: AB100507
Price: 109000 JPY
Availability: InStock

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(4 Publications)

Human CD105 ELISA Kit (Endoglin) is a Sandwich ELISA for the measurement of Human CD105 (Endoglin) in Human in Biofluids, Cell Culture Media samples.

別名を表示する

CD105, END, ENG, Endoglin

1 Images

Sandwich ELISA - Human CD105 ELISA Kit (Endoglin) (AB100507)

sELISA

Supplier Data

Sandwich ELISA - Human CD105 ELISA Kit (Endoglin) (AB100507)

Representative Standard Curve using ab100507.

Key facts

検出方法

Colorimetric

サンプルタイプ

Plasma, Cell culture supernatant, Serum

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

< 10 pg/mL

検出範囲

12.35 - 3000 pg/mL

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Abcam's CD105 (Endoglin) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD105 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human CD105 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD105 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD105 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD105 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimisation may be required with urine samples.

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回収率

[ { "sample": "Cell culture supernatant", "range": "87 - 110 %", "average": "= 96.92" }, { "sample": "Serum", "range": "111 - 130 %", "average": "= 120.1" }, { "sample": "Plasma", "range": "94 - 132 %", "average": "= 116.7" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100507-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100507-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CD105 Microplate (12 x 8 well strips)", "number":"AB100507-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100507-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100507-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100507-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human CD105 Standard (lyophilized)", "number":"AB100507-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human CD105", "number":"AB100507-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100507-CMP02", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD105 also known as endoglin or the CD105 marker is a transmembrane glycoprotein with a molecular weight of approximately 180 kDa. It is a component of the TGF-beta receptor complex and exists in endothelial cells where it is abundantly expressed. Expression of CD105 is higher in proliferating cells particularly in the vasculature. You can also find it in tissues involved in the formation and remodeling of blood vessels such as during angiogenesis.

Biological function summary

Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.

Pathways

CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.

CD105 has links to hereditary hemorrhagic telangiectasia (HHT) and certain cancers. In HHT mutations in the endoglin gene alter vascular structure leading to the formation of abnormal blood vessels. Oncologically overexpression of CD105 is present in tumor angiogenesis aiding in the progression of certain cancers. Other proteins like VEGF and TGF-beta closely interact with endoglin influencing disease progression and presenting potential targets for therapeutic intervention.

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製品プロトコール

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ターゲットの情報

Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed : 21737454, PubMed : 23300529). Required for normal structure and integrity of adult vasculature (PubMed : 7894484). Regulates the migration of vascular endothelial cells (PubMed : 17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed : 1692830). Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529, PubMed : 8370410). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529).

See full target information ENG

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Clinical cancer research : an official journal of the American Association for Cancer Research 29:3744-3758 PubMed37432984

2023

Endoglin, a Novel Biomarker and Therapeutical Target to Prevent Malignant Peripheral Nerve Sheath Tumor Growth and Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa González-Muñoz,Angela Di Giannatale,Susana García-Silva,Vanesa Santos,Sara Sánchez-Redondo,Claudia Savini,Osvaldo Graña-Castro,Carmen Blanco-Aparicio,Suzanne Fischer,Olivier De Wever,Edgar Creus-Bachiller,Sara Ortega-Bertran,David J Pisapia,Jose L Rodríguez-Peralto,Juana Fernández-Rodríguez,Cleofé Romagosa Pérez-Portabella,Rita Alaggio,Maria Serena Benassi,Laura Pazzaglia,Katia Scotlandi,Nancy Ratner,Kaleb Yohay,Charles P Theuer,Héctor Peinado

Molecules (Basel, Switzerland) 26: PubMed33440679

2021

Zeaxanthin-Rich Extract from Superfood Selectively Modulates the Cellular Adhesion and MAPK Signaling in Melanoma versus Normal Skin Cells In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Diana Cenariu,Eva Fischer-Fodor,Adrian Bogdan Țigu,Andrea Bunea,Piroska Virág,Maria Perde-Schrepler,Vlad-Alexandru Toma,Andrei Mocan,Ioana Berindan-Neagoe,Adela Pintea,Gianina Crișan,Mihai Cenariu,Alma Maniu

Scientific reports 8:2425 PubMed29402914

2018

Neuroprotection of the hypoxic-ischemic mouse brain by human CD117CD90CD105 amniotic fluid stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Michelangelo Corcelli,Kate Hawkins,Filipa Vlahova,Avina Hunjan,Kate Dowding,Paolo De Coppi,Anna L David,Donald Peebles,Pierre Gressens,Henrik Hagberg,Mariya Hristova,Pascale V Guillot

PloS one 10:e0131013 PubMed26121675

2015

Maternal Serum Meteorin Levels and the Risk of Preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

María F Garcés,Elizabeth Sanchez,Luisa F Cardona,Elkin L Simanca,Iván González,Luis G Leal,José A Mora,Andrés Bedoya,Juan P Alzate,Ángel Y Sánchez,Javier H Eslava-Schmalbach,Roberto Franco-Vega,Mario O Parra,Ariel I Ruíz-Parra,Carlos Diéguez,Rubén Nogueiras,Jorge E Caminos

View all publications

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Human CD105 ELISA Kit (Endoglin)