Human CCL23 ELISA Kit
Human CCL23 ELISA Kit
- Recombinant
- SimpleStep
- 詳細を見る
Be the first to review this product! Submit a review
|
(0 Publication)
Human CCL23 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CCL23 with a sensitivity of 1.54 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
別名を表示する
MIP3, MPIF1, SCYA23, CCL23, C-C motif chemokine 23, CK-beta-8, Macrophage inflammatory protein 3, Myeloid progenitor inhibitory factor 1, Small-inducible cytokine A23, CKB-8, MIP-3, MPIF-1
- sELISA
Supplier Data
Sandwich ELISA - Human CCL23 ELISA Kit (AB216169)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP3 concentration was determined to be 797 pg/mL with a range of 229 – 1,754 pg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human CCL23 ELISA Kit (AB216169)
Interpolated concentrations of native MIP3 in human serum and plasma samples.
The concentrations of MIP3 were measured in duplicates, interpolated from the MIP3 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 50% and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP3 concentration was determined to be 698 pg/mL in serum, 621 pg/mL in plasma (citrate), 689 pg/mL in plasma (heparin) and 592 pg/mL in plasma (EDTA).
- sELISA
Supplier Data
Sandwich ELISA - Human CCL23 ELISA Kit (AB216169)
Interpolated concentrations of native MIP3 in human synovial fluid and THP-1 cell culture supernatant samples.
The concentrations of MIP3 were measured in duplicates, interpolated from the MIP3 standard curves and corrected for sample dilution. Undiluted samples are as follows : synovial fluid 25% and THP-1 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP3 concentration was determined to be 182 pg/mL in synovial fluid and 130 pg/mL in THP-1 supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Human CCL23 ELISA Kit (AB216169)
Representative image of Human CCL23 ELISA Kit (ab216169)
Components shown from left to right :
- Sample diluent NS
- 10X Human CCL23 Detector Antibody
- Human CCL23 Lyophilised Purified Protein (2 vials)
- Human CCL23 Capture Antibody (Lyophilised)
- Stop Solution
- TMB solution
- 10X Wash Buffer PT (ab206977)
- Antibody Diluent 5BI
SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.
Plate Seals - shown underneath bottles / vials.
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit
- sELISA
Supplier Data
Sandwich ELISA - Human CCL23 ELISA Kit (AB216169)
Example of human MIP3 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
組合せ製品
Reactivity data
製品の詳細
Human CCL23 ELISA Kit (ab216169) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL23 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CCL23 with 1.54 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CCL23 (Macrophage inflammatory protein 3) is a chemokine involved in the recruitment of monocytes, neutrophils and resting T cells. CCL23 functions by inhibition myeloid progenitor cell proliferation. CCL23 is found at high levels in the synovial fluid of patients with rheumatoid arthritis.
精度
回収率
製品内容
出荷温度及び保存条件
出荷温度
短期保存温度
長期保存温度
保管に関する情報
補足情報
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CCL23 functions in the recruitment of immune cells especially eosinophils and monocytes to areas where immune responses are necessary. This chemokine is not known to be part of a larger protein complex but its ability to interact with specific receptors on cell surfaces makes it integral to immune signaling. During immune activation CCL23 contributes to the inflammatory cascade influencing both acute and chronic inflammatory responses.
Pathways
The signaling functions of CCL23 integrate within the chemokine signaling pathway and the broader immune response pathway. CCL23 interacts closely with proteins such as CCR1 which acts as its receptor and influences cellular responses via this receptor-mediated signaling. Additionally CCL23 shares mechanistic parallels with other chemokines like CCL22 though they may attract different subsets of cells they participate in coordinated immune activities.
ターゲットの情報
Abcam product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com