AB314600

Human CCL1 ELISA Kit

SimpleStep
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Human CCL1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CCL1 with a sensitivity of 1.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

SCYA1, CCL1, C-C motif chemokine 1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309

2 Images

Sandwich ELISA - Human CCL1 ELISA Kit (AB314600)

sELISA

Supplier Data

Sandwich ELISA - Human CCL1 ELISA Kit (AB314600)

Interpolated concentrations of human CCL1 in treated and untreated Jurkat supernatant

Interpolated concentration of native CCL1 was measured in duplicate at different sample concentrations. Undiluted samples are 50% Jurkat supernatant (treated with 3µg/ml PHA + 10ng/ml TPA) and untreated Jurkat supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human CCL1 ELISA Kit (AB314600)

Example of human CCL1 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 1.4 pg/mL

検出範囲

4.688 - 300 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human CCL1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL1 protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human CCL1 with 1.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精度

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.41" } ]

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回収率

[ { "sample": "Serum", "range": "79 - 92 %", "average": "= 87" }, { "sample": "Citrate plasma", "range": "91 - 94 %", "average": "= 92" }, { "sample": "EDTA Plasma", "range": "85 - 96 %", "average": "= 91" }, { "sample": "Heparin Plasma", "range": "82 - 96 %", "average": "= 91" }, { "sample": "Cell culture supernatant", "range": "93 - 107 %", "average": "= 100" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The CCL1 protein also known as I-309 protein is a cytokine that plays a significant role in the immune system. Its molecular mass is approximately 10 kDa. CCL1 is secreted by activated T cells and is found extensively in the thymus. It binds to the CCR8 receptor which suggests its involvement in immune signaling. This protein belongs to the CC chemokine family which is characterized by the presence of two adjacent cysteines near the amino terminus.

Biological function summary

CCL1 influences immune cell trafficking and activation. It attracts monocytes T lymphocytes and some types of dendritic cells to sites of inflammation. Although it is not part of a large protein complex CCL1 works with other chemokines to modulate the immune response contributing to both innate and adaptive immune mechanisms. Its expression may increase or decrease depending on the immune needs of the body.

Pathways

Studies show that CCL1 participates in the chemokine signaling pathway and the effector T-cell pathway. Within the chemokine signaling pathway CCL1 interacts with other chemokines like CCL17 and proteins involved in T-cell migration. This pathway helps coordinate the movement of immune cells to sites of inflammation or injury ensuring an effective immune response.

Research links CCL1 to conditions such as asthma and rheumatoid arthritis. In asthma CCL1 levels influence the recruitment of immune cells to the lungs contributing to inflammation and airway hyper-responsiveness. Similarly in rheumatoid arthritis its interaction with CCR8 has been associated with synovial inflammation. These diseases indicate that CCL1’s role in immune cell migration can have pathological consequences when dysregulated.

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製品プロトコール

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ターゲットの情報

Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8.

See full target information CCL1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 14: PubMed39063997

2024

The Role of Pro-Inflammatory Chemokines CCL-1, 2, 4, and 5 in the Etiopathogenesis of Type 2 Diabetes Mellitus in Subjects from the Asir Region of Saudi Arabia: Correlation with Different Degrees of Obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Muzaffar Mir,Jaber Alfaifi,Shahzada Khalid Sohail,Syeda Fatima Rizvi,Md Tanwir Akhtar,Mushabab Ayed Abdullah Alghamdi,Rashid Mir,Javed Iqbal Wani,Zia Ul Sabah,Fahad A Alhumaydhi,Fahad Alremthi,AbdulElah Al Jarallah AlQahtani,Muffarah Hamid Alharthi,Masoud Ishag Elkhalifa Adam,Imadeldin Elfaki,Hany M A Sonpol

View all publications

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websiteProtocolBooklet

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Abcam product promise

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Human CCL1 ELISA Kit