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  1. Link

    products/elisa-kits/human-carcino-embryonic-antigen-elisa-kit-cea-ab99992.pdf

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Human Carcino Embryonic Antigen ELISA Kit (CEA) (ab99992)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1)Q&A (6)References (11)

Product price, shipping and contact information

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Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Sandwich ELISA - Human CEA (ab99992)
  • Typical Standard Curve
  • Typical Standard Curve

Key features and details

  • Sensitivity: 0.2 ng/ml
  • Range: 0.343 ng/ml - 250 ng/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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関連製品

製品の概要

  • 製品名

    Human Carcino Embryonic Antigen ELISA Kit (CEA)
  • 検出方法

    Colorimetric
  • サンプルの種類

    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    < 0.2 ng/ml
  • 検出範囲

    0.343 ng/ml - 250 ng/ml
  • 添加回収試験

    > 100 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 116.7 93% - 136%
    Serum 145.6 135% - 156%
    Plasma 103.8 82% - 124%
  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Human
  • 製品の概要

    Abcam’s Carcinoembryonic Antigen (CEA) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CEA in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human CEA coated on a 96-well plate. Standards and samples are pipetted into the wells and CEA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CEA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CEA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get results in 90 minutes with Human Carcinoembryonic Antigen ELISA Kit (CD66e) (ab183365) from our SimpleStep ELISA® range.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    1,000X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human CEA 2 vials
    CEA Microplate (12 x 8 well strips) 1 unit
    Recombinant Human CEA Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 研究分野

    • Cancer
    • Tumor immunology
    • Tumor-associated antigens
    • Cancer
    • Tumor biomarkers
    • Tumor antigens
    • Microbiology
    • Protein
    • Human Protein
    • Receptor
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Tumor biomarkers ELISA kits
  • 関連性

    Carcino Embryonic Antigen (CEA) is synthesised during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).
  • 細胞内局在

    Cell Membrane
  • 別名

    • Carcinoembryonic antigen
    • Carcinoembryonic antigen related cell adhesion molecule 5
    • CD66e
    • CD66e antigen
    • CEA
    • CEACAM 5
    • CEACAM5
    • DKFZp781M2392
    • Meconium antigen 100
    see all
  • 参照データベース

    • Entrez Gene: 1048 Human
    • SwissProt: P06731 Human

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab99992の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Sandwich ELISA
    Use at an assay dependent concentration.
    特記事項
    Sandwich ELISA
    Use at an assay dependent concentration.

    画像

    • Sandwich ELISA - Human CEA (ab99992)
      Sandwich ELISA - Human CEA (ab99992)

      CEA measured in biological fluids showing quantity (ng) per mL of tested sample

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab99992.

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab99992.

    プロトコール

    • Protocol Booklet

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (11)

    ab99992 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab99992 は 11 報の論文で使用されています。

    • Ma J  et al. Detection Rate and Prognosis of Lymph Nodes in Gastric Cancer Using Nano Carbon Combined with In Vitro Anatomical Sorting. Comput Intell Neurosci 2022:4540176 (2022). PubMed: 36052030
    • Joshi S  et al. Simple diagnosis of cancer by detecting CEA and CYFRA 21-1 in saliva using electronic sensors. Sci Rep 12:15315 (2022). PubMed: 36097151
    • Alhetheel A  et al. Differential expression of carcinoembryonic antigen-related cell adhesion molecule-5 (CEACAM5) and dipeptidyl peptidase-4 (DPP4) with detection of Middle East respiratory syndrome-coronavirus in peripheral blood. J Infect Public Health 15:1315-1320 (2022). PubMed: 36279687
    • Kang C  et al. Construction and Validation of a Lung Cancer Diagnostic Model Based on 6-Gene Methylation Frequency in Blood, Clinical Features, and Serum Tumor Markers. Comput Math Methods Med 2021:9987067 (2021). PubMed: 34257703
    • Gökçay Canpolat A  et al. Calcitonin levels in washout samples vs. cytology in the detection of malignant lymph node metastasis in recurrent medullary thyroid cancer. Turk J Med Sci 51:3061-3066 (2021). PubMed: 34519193
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-7 of 7 Abreviews or Q&A

    CEA concentration of normal or lung cancer sera

    Average Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    Difference between normal group and lung adenocarcinoma (ADC) group was very clear.

    But the absolute values measured by ab99992 were remarkably higher than clinical tests in hospital or CRO companies.

    To make matters worse, when we increased the dilution factor (x2, x5, or x10 dilution), the absolute values of CEA also gradually increased.

    Hence this kit can be used for only "relative quantification" studies.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Nov 05 2013

    Question

    Dear technical team,

    Our customer purchased ab99992 last November and she opened this kit today to test.
    And she confirmed there are 5μl of item G not 200μl so she would like to know if you have alternative item.
    Please let me know if you have information.
    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Jul 18 2012

    Answer

    Thank you for your inquiry.

    I can confirm that the kit should contain 8 ul of HRP-Streptavidin Concentrate (Item G). Please tell your customer to spin down the vial. The kit was ordered in November 2011 and we did change the amount of HRP-Streptavidin Concentrate (Item G) to 200ul in March 2012.

    The protocol booklet that your customer received with this kit will detail that HRP-Streptavidin Concentrate (Item G) is 8ul.

    I would also like to mention that the Abpromise guarantee period for our products is 6 month and therefore we suggest to check the products shortly after they delivered to avoid disappointment.

    I attached the correct protocol for the kit your customer received to this email for your information.

    I hope this will be helpful to your customer and wish you both a

    Read More

    Abcam Scientific Support

    Answered on Jul 18 2012

    Question

    Can you tell me which antibody is used as a capture antibody for the CEA ELISA # ab99992 that you market?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 20 2012

    Answer

    Thank you for your inquiry.

    The capture antibody in this kit is supplied by an outside lab and unfortunately they were not willing to provide me with any other details about it. They did want to know if you were interested in it to follow up on some data from the ELISA assay? Perhaps they would be willing to offer it for sale, but I'm not sure if that's what you wanted.

    I hope this information helps. Please contact us with any other question.

    Read More

    Abcam Scientific Support

    Answered on Jul 20 2012

    Question

    Sorry for bothering you again, but I was wondering if you happen to know if there is an anti-CEA antibody available in you company that is HRP-conjugated? In the report of a PhD student before me I read that she purchased anti-CEA coated with HRP from Abcam. I can't seem to find that on the site though.



    Thanks in advance

    Read More

    Abcam community

    Verified customer

    Asked on Feb 27 2012

    Answer

    Thank you for your new enquiry and continuous interest in our products.

    I have conducted a search for you and we have FITC and PE-conjugated antibodies but not HRP-linked primary antibody against CEA.

    However, we have two CEA ELISA kits in our catalogue (ab99992, ab108635) which you may be interested. Please take a look at the on-line datasheet for further information and le me know if you have any further questions.

    Have a nice day!

    Read More

    Abcam Scientific Support

    Answered on Feb 27 2012

    Question

    is there any information regarding detection of mouse proteinwith this kit?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 26 2012

    Answer

    Unfortunately there is no cross reactivity data available testing the Human CEA ELISA Kit ab99992 with mouse. If it helps though, the capture antibody is a mouse monoclonal and reacts with the protein determinant belonging to the “gold” epitope group 4. The detection antibody is a mouse monoclonal antibody reacting to the “gold” epitope group 5.

    We do have a testing discount promotion which you may be interested in as you will be testing mouse with this ELISA kit. Please see below for a description:

    ===================

    To our knowledge, ab99992 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab99992 now, test it in mouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free ELISA kit or 20% off a primary antibody or protein product.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab99992 in mouse. I will then send a discount code. This code must be issued before purchasing ab99992 so please wait for my reply before ordering.

    2. Purchase ab99992 either by phone, fax, or online (www.abcam.com).

    3. Test it in mouse.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any ELISA kit or 20% off a primary antibody or protein product and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab99992 turns out to be unsuitable for 1ELISA kit or 20% off a primary antibody or protein product, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Jan 26 2012

    Question

    I am interested in ab99992 but also want to compare it to a new technology I am developing and am wondering if I may also purchase the primary antibody used in that kit although it is not in your catalog.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 23 2011

    Answer

    At the moment we do not sell kit components separately, this is because that we only produce enough reagents for use with the kit and do not have any spare to sell separately. We may in future add the antibody in its own right but for now it can only be purchased as part of a kit.

    Read More

    Abcam Scientific Support

    Answered on Nov 23 2011

    Question

    Dear technical team, Our customer would like to purchase CEA ELISA kit and alpha Fetoprotein ELISA Kit. She has searched ab108631, ab108838, ab108635, ab99992 and asked well plate type.   Is it strip type or one plate type?  

    Read More

    Abcam community

    Verified customer

    Asked on Nov 01 2011

    Answer

    Thank you for contacting us. I can confirm that all the mentioned kits (ab99992, ab108631, ab108635, ab108838) come with a 96-well strip plate (12x8).  Each strip (12 total) has 8 wells so the customer can use a strip at a time if they wish. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Nov 01 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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