Human Carbonic Anhydrase 9/CA9 ELISA Kit (ab289905)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.141 pg/ml
- Range: 19.531 pg/ml - 1250 pg/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Carbonic Anhydrase 9/CA9 ELISA Kit
Carbonic Anhydrase 9/CA9 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Supernatant 8 9.8% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Supernatant 3 9.7% -
サンプルの種類
Cell culture supernatant -
アッセイタイプ
Sandwich (quantitative) -
検出感度
>= 3.141 pg/ml -
検出範囲
19.531 pg/ml - 1250 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 109 94% - 120% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human Carbonic Anhydrase 9/CA9 ELISA Kit (ab289905) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Carbonic Anhydrase 9/CA9 in cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Carbonic Anhydrase 9/CA9 with 3.141 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human Carbonic Anhydrase 9/CA9 Capture Antibody 1 x 600µl 10X Human Carbonic Anhydrase 9/CA9 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Carbonic Anhydrase 9/CA9 Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. -
組織特異性
Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. -
配列類似性
Belongs to the alpha-carbonic anhydrase family.
Contains 1 alpha-carbonic anhydrase domain. -
翻訳後修飾
Asn-346 bears high-mannose type glycan structures. -
細胞内局在
Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus. - Information by UniProt
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別名
- CA-IX
- CA9
- CAH9_HUMAN
see all -
参照データベース
- Entrez Gene: 768 Human
- Omim: 603179 Human
- SwissProt: Q16790 Human
- Unigene: 63287 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab289905の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Example of human Carbonic Anhydrase 9/CA9 standard curve in Sample Diluent NS.
Example of human Carbonic Anhydrase 9/CA9 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of human Carbonic Anhydrase 9/CA9 in HT-29 Supernatant.Interpolated concentration of native Carbonic Anhydrase 9/CA9 was measured in duplicate at different sample concentrations. Undiluted samples are 20% HT-29 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Human Carbonic Anhydrase 9/CA9 competitor standard curve comparison.Standard curve comparison between Human Carbonic Anhydrase 9/CA9 SimpleStep ELISA kit (ab289905) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows comparable sensitivity.
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Sandwich ELISA - Human Carbonic Anhydrase 9/CA9 ELISA Kit (ab289905)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Human Human Carbonic Anhydrase 9/CA9 ELISA Kit (ab289905) -
Sandwich ELISA - Human Carbonic Anhydrase 9/CA9 ELISA Kit (ab289905)To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab289905 は論文での使用が確認できていません。