AB303755

Human Carbonic Anhydrase 1/CA1 ELISA Kit

Recombinant
SimpleStep
詳細を見る

Be the first to review this product! Submit a review

(0 Publication)

Human Carbonic Anhydrase 1/CA1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Carbonic Anhydrase 1/CA1 with a sensitivity of 19.537 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

Carbonic anhydrase 1, Carbonate dehydratase I, Carbonic anhydrase B, Carbonic anhydrase I, Cyanamide hydratase CA1, CAB, CA-I, CA1

3 Images

Sandwich ELISA - Human Carbonic Anhydrase 1/CA1 ELISA Kit (AB303755)

sELISA

Supplier Data

Sandwich ELISA - Human Carbonic Anhydrase 1/CA1 ELISA Kit (AB303755)

Interpolated concentrations of human Carbonic Anhydrase 1/CA1 in serum, plasma (citrate), plasma (EDTA), plasma (heparin), and saliva

Interpolated concentration of native Carbonic Anhydrase 1/CA1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 800, plasma – citrate 1 : 800, plasma – EDTA 1 : 800, plasma – heparin 1 : 800, and saliva 1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Carbonic Anhydrase 1/CA1 ELISA Kit (AB303755)

Example of human Carbonic Anhydrase 1/CA1 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Carbonic Anhydrase 1/CA1 ELISA Kit (AB303755)

Interpolated concentrations of Carbonic Anhydrase 1/CA1 in normal human serum donors.

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Carbonic Anhydrase 1/CA1 concentration was determined to be 2,801 ng/mL with a range of 1,654 – 5,612 ng/mL.

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Saliva

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 19.537 pg/mL

検出範囲

62.5 - 4000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

製品の詳細

Human Carbonic Anhydrase 1/CA1 SimpleStep ELISA® kit (ab303755) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Carbonic Anhydrase 1/CA1 protein in serum, plasma (hep), plasma (cit), plasma (EDTA) and saliva. Quantitate Human Carbonic Anhydrase 1/CA1 (UBB+1) with 19.537 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style

left-column

精度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]

style

left-column

回収率

[ { "sample": "Serum", "range": "102 - 116 %", "average": "= 107" }, { "sample": "Citrate plasma", "range": "90 - 112 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "99 - 109 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "91 - 109 %", "average": "= 102" }, { "sample": "Saliva", "range": "91 - 109 %", "average": "= 103" } ]

style

left-column

製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab303755-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB303755-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB303755-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB303755-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB303755-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB303755-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB303755-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB303755-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Carbonic Anhydrase 1/CA1 Capture Antibody", "number":"AB303755-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Carbonic Anhydrase 1/CA1 Lyophilized Recombinant Protein", "number":"AB303755-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Carbonic Anhydrase 1/CA1 Detector Antibody", "number":"AB303755-CMP03", "productcode":"" } ] } } }

style

left-column

出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

style

left-column

style

left-column

製品プロトコール

style

left-column

ターゲットの情報

Catalyzes the reversible hydration of carbon dioxide (PubMed : 10550681, PubMed : 16506782, PubMed : 16686544, PubMed : 16807956, PubMed : 17127057, PubMed : 17314045, PubMed : 17407288, PubMed : 18618712, PubMed : 19186056, PubMed : 19206230). Can hydrate cyanamide to urea (PubMed : 10550681).

See full target information Carbonic anhydrase 1

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Carbonic Anhydrase 1/CA1 ELISA Kit