AB309427

Human beta-Glucuronidase ELISA Kit

Name: Human beta-Glucuronidase ELISA Kit (ab309427) | Abcam
Brand: Abcam Limited
SKU: AB309427
Price: 135000 JPY
Availability: InStock

Be the first to review this product! Submit a review

(0 Publication)

Human beta-Glucuronidase ELISA Kit is a Sandwich ELISA for the measurement of Human beta-Glucuronidase in Human in Biofluids, Cell Culture Media samples.

別名を表示する

Beta-glucuronidase, Beta-G1, GUSB

1 Images

Sandwich ELISA - Human beta-Glucuronidase ELISA Kit (AB309427)

sELISA

Supplier Data

Sandwich ELISA - Human beta-Glucuronidase ELISA Kit (AB309427)

Example of standard curve using ab309427. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, Plasma, Cell culture supernatant

交差種

Human

アッセイタイプ

Sandwich

Quantitative

検出感度

= 0.18 ng/mL

検出範囲

0.18 - 40 ng/mL

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

製品の詳細

Human beta-Glucuronidase ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human beta-Glucuronidase in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human beta-Glucuronidase coated on a 96-well plate

style

left-column

回収率

[ { "sample": "Serum", "range": "104 - 129 %", "average": "= 111.7" }, { "sample": "Plasma", "range": "104 - 132 %", "average": "= 118.5" }, { "sample": "Cell culture media", "range": "80 - 125 %", "average": "= 108.7" } ]

style

left-column

製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309427-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309427-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309427-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human beta-Glucuronidase/GUSB Protein Standard", "number":"AB309427-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"900X HRP-Streptavidin", "number":"AB309427-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human beta-Glucuronidase/GUSB Antibody-coated ELISA Plate", "number":"AB309427-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309427-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309427-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human beta-Glucuronidase/GUSB Detection Antibody", "number":"AB309427-CMP09", "productcode":"" } ] } } }

style

left-column

出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Beta glucuronidase also known as GUSB is an enzyme with several well-known names including b glucuronidase b-glucuronidase and beta-glucuronidase. It is a hydrolase enzyme with a molecular mass around 75 kDa. The enzyme is widely expressed in lysosomes of various tissues such as liver spleen and kidney. Mechanically beta-glucuronidase cleaves beta-D-glucuronic acid residues from glycosaminoglycans steroids and hormones an essential step for the degradation of glycosaminoglycans.

Biological function summary

Beta glucuronidase performs significant roles in cellular metabolism and molecule recycling. This enzyme aids in the breakdown and recycling of complex carbohydrates. By facilitating hydrolysis it helps in maintaining cellular homeostasis. Beta glucuronidase is not part of a complex; it functions independently within lysosomes. Its activity is vital for recycling endocytosed material and managing cellular turnover.

Pathways

Beta glucuronidase plays an important role in the lysosomal degradation pathway. This enzyme aids in glycosaminoglycan catabolism a process important for cellular waste disposal and recycling. Its activity connects with several proteins in the pathway notably those involved in lysosomal function such as Hexosaminidase. Beta glucuronidase's enzymatic function further integrates into the broader context of carbohydrate metabolism pathways enabling efficient breakdown of large carbohydrate molecules.

Mutations or deficiencies in beta glucuronidase lead to mucopolysaccharidosis type VII also called Sly syndrome. This lysosomal storage disorder results in the accumulation of undegraded glycosaminoglycans causing developmental delay skeletal abnormalities and other systemic issues. Additionally beta glucuronidase contributes to cancer biology where altered expressions can influence tumor progression. Proteins like glucocerebrosidase may interact indirectly impacting the disease's pathophysiology.

style

left-column

製品プロトコール

style

left-column

ターゲットの情報

Plays an important role in the degradation of dermatan and keratan sulfates.

See full target information GUSB

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human beta-Glucuronidase ELISA Kit