AB227900

Human BAG3 ELISA Kit

Recombinant
SimpleStep
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Human BAG3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human BAG3 with a sensitivity of 11.7 pg/well. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

BIS, BAG3, BAG family molecular chaperone regulator 3, BAG-3, Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis, Docking protein CAIR-1

3 Images

ELISA

Supplier Data

ELISA - Human BAG3 ELISA Kit (AB227900)

sELISA

Supplier Data

Sandwich ELISA - Human BAG3 ELISA Kit (AB227900)

Example of human Bag3 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human BAG3 ELISA Kit (AB227900)

Interpolated concentrations of native Bag3 in HEK293T cell extract based on a 100 µg/mL extract load and human heart tissue extract based on a 150 μg/mL extract load.

The concentrations of Bag3 were measured in duplicate and interpolated from the Bag3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Bag3 concentration was determined to be 2497 pg/mL in HEK293T cell extract and 2743 pg/mL in human heart tissue extract.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture extracts, Tissue Extracts

交差種

Human

アッセイタイプ

Sandwich (quantitative)

検出感度

= 11.7 pg/well

検出範囲

39.1 - 2500 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human BAG3 ELISA Kit ab227900 is a rapid single-wash 90-min Sandwich ELISA to measure Human BAG3 in cell culture extracts, tissue extracts. This SimpleStep sensitivity is 11.7 pg/weLL.

How the assay works

Human BAG3 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human BAG3 ELISA Kit ab227900 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Bag3 antibody [EPR20207-4] - BSA and Azide free (Capture) ab244958
- Anti-Bag3 antibody [EPR20207-109] - BSA and Azide free (Detector) ab244958

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "HEK-293T", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" }, { "reproducibilityType": "Intra", "sample": "HEK-293T", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]

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回収率

[ { "sample": "Cell culture extracts", "range": "93 - 99 %", "average": "= 97" }, { "sample": "Tissue Extracts", "range": "103 - 105 %", "average": "= 104" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The Bcl-2-associated athanogene 3 commonly known as Bag3 is a protein with a molecular weight of approximately 74 kDa. It plays an important role in apoptosis regulation and stress responses by interacting with heat shock proteins. Bag3 is expressed in various tissues including the heart skeletal muscles and certain types of neurons. It functions mechanically by binding to chaperones like Hsp70 modulating their activity and helping in protein folding and degradation processes.

Biological function summary

Bag3 contributes to cell survival by assisting in protein homeostasis and mitigating stress-related cellular damage. It acts as a co-chaperone and forms part of complexes with Hsp70 HspB8 and CHIP among others. These interactions enhance the cellular capacity to handle misfolded proteins preventing the aggregation that could lead to cell toxicity. In muscle cells Bag3 plays a role in maintaining structural integrity by participating in the assembly of myofibrils critical for muscle function.

Pathways

The protein is involved in autophagy and apoptosis regulation. It actively participates in the ubiquitin-proteasome system and chaperone-mediated autophagy collaborating closely with proteins like HspB8 and Hsp70. The integration of Bag3 within these pathways highlights its involvement in removing damaged proteins and preserving cellular function during stress conditions. Additionally Bag3 contributes to the regulation of the NF-kB pathway which is involved in the immune response and inflammation.

Mutations or dysregulation of Bag3 associate with conditions such as myopathy and certain cancers. In myopathy aberrations in Bag3 affect muscle resilience and repair leading to structural muscle defects. In cancer Bag3's role in inhibiting apoptosis promotes tumor cell survival making it a target for therapeutic interventions. The protein has connections with p53 in cancer contexts where it influences cell death and survival decisions. Understanding Bag3's interactions and functions in these diseases opens new opportunities for developing treatments targeting these pathways.

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製品プロトコール

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ターゲットの情報

Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70 (PubMed : 27474739, PubMed : 9873016). Has anti-apoptotic activity (PubMed : 10597216). Plays a role in the HSF1 nucleocytoplasmic transport (PubMed : 26159920).

See full target information BAG3

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websiteProtocolBooklet

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Human BAG3 ELISA Kit