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SimpleStep

Human BACE2 ELISA Kit (ab289899)

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Example of human BACE2 standard curve in 1X Cell Extraction Buffer PTR
  • Interpolated concentrations of human BACE2 in HeLa and cerebellum extract.
  • Human BACE2 ELISA Kit (ab289899)
  • Human BACE2 ELISA Kit (ab289899)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 11.2 pg/ml
  • Range: 54.688 pg/ml - 3500 pg/ml
  • Sample type: Cell culture extracts, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

製品の概要

  • 製品名

    Human BACE2 ELISA Kit
    BACE2 キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Extract 8 11.2%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Extract 3 12.7%

  • サンプルの種類

    Cell culture extracts, Tissue Extracts

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    >= 11.2 pg/ml

  • 検出範囲

    54.688 pg/ml - 3500 pg/ml

  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Tissue Extracts 102 99% - 104%
    Cell Lysate 99 87% - 109%

  • 全工程の試験時間

    1h 30m

  • ステップ

    One step assay

  • 種交差性

    交差種: Human

  • 製品の概要

    Human BACE2 ELISA Kit (ab289899) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human BACE2 in cell lysate samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BACE2 with 11.2 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


    -Single-wash protocol reduces assay time to 90 minutes or less
    -High sensitivity, specificity and reproducibility from superior antibodies
    -Fully validated in biological samples
    -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • 特記事項

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

  • 保存方法

    Please refer to protocols.
  • 内容 1 x 96 tests
    10X Human BACE2 Capture Antibody 1 x 600µl
    10X Human BACE2 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPR2 1 x 6ml
    Human BACE2 Lyophilized Recombinant Protein 2 vials
    Plate Seal 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • 研究分野

  • 機能

    Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672.

  • 組織特異性

    Brain. Present in neurons within the hippocampus, frontal cortex and temporal cortex (at protein level). Expressed at low levels in most peripheral tissues and at higher levels in colon, kidney, pancreas, placenta, prostate, stomach and trachea. Expressed at low levels in the brain. Found in spinal cord, medulla oblongata, substantia nigra and locus coruleus. Expressed in the ductal epithelium of both normal and malignant prostate.

  • 配列類似性

    Belongs to the peptidase A1 family.

  • 翻訳後修飾

    Undergoes autoproteolytic cleavage.
    Glycosylated.

  • 細胞内局在

    Membrane. Golgi apparatus. Endoplasmic reticulum. Endosome. Cell surface.
  • Information by UniProt

  • 別名

    • ALP 56
    • ALP56
    • Asp 1
    • ASP1
    • ASP21
    • Aspartic Like Protease 56kDa
    • Aspartic-like protease 56 kDa
    • Aspartyl protease 1
    • BACE 2
    • Bace2
    • BACE2_HUMAN
    • Beta Secretase 2
    • Beta Site Amyloid Beta A4 Precursor Protein Cleaving Enzyme 2
    • Beta-secretase 2
    • Beta-site amyloid precursor protein cleaving enzyme 2
    • Beta-site APP cleaving enzyme 2
    • Down region aspartic protease
    • Downs Syndrome Region Aspartic Protease
    • DRAP
    • Memapsin 1
    • Memapsin-1
    • Membrane associated aspartic protease 1
    • Membrane-associated aspartic protease 1
    • Theta-secretase
    see all

  • 参照データベース

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab289899の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Sandwich ELISA
    Use at an assay dependent concentration.
    特記事項
    Sandwich ELISA
    Use at an assay dependent concentration.

    画像

    • Example of human BACE2 standard curve in 1X Cell Extraction Buffer PTR
      Example of human BACE2 standard curve in 1X Cell Extraction Buffer PTR

      Example of human BACE2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of human BACE2 in HeLa and cerebellum extract.
      Interpolated concentrations of human BACE2 in HeLa and cerebellum extract.

      Interpolated concentration of native BACE2 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: HeLa 1,000 µg/mL and cerebellum 500 µg/mL.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

    • Human BACE2 ELISA Kit (ab289899)
      Human BACE2 ELISA Kit (ab289899)
    • Human BACE2 ELISA Kit (ab289899)
      Human BACE2 ELISA Kit (ab289899)

    参考文献 (0)

    ab289899 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab289899 は論文での使用が確認できていません。

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