AB316917

Human ATF2 (phospho T71) ELISA Kit

SimpleStep
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Human ATF2 (phospho T71) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ATF2 (phospho T71) with a sensitivity of 4.573 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

CREB2, CREBP1, ATF2, Cyclic AMP-dependent transcription factor ATF-2, cAMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2, HB16, cAMP response element-binding protein CRE-BP1, CREB-2, cAMP-responsive element-binding protein 2

3 Images

Sandwich ELISA - Human ATF2 (phospho T71) ELISA Kit (AB316917)

sELISA

Supplier Data

Sandwich ELISA - Human ATF2 (phospho T71) ELISA Kit (AB316917)

Interpolated concentration of native ATF2 (phospho T71) was measured in duplicate at different sample concentrations. Undiluted samples are 500 μg/mL HeLa cell extract (treated with 10 mM Anisomycin for 30 min). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human ATF2 (phospho T71) ELISA Kit (AB316917)

HeLa cells were treated with 10 mM anisomycin for 30 minutes, scraped and buffer exchanged into PBS + 20 mM NaF then followed by protein extraction. Mock control HeLa cells accompanied cell treatment. Concentrations of ATF2 (phospho T71) were measured in duplicate at 500 μg/mL load and interpolated against the standard curve. Results show the mean ATF2 (phospho T71) was not detected (ND) in the control samples compared to 1,645 pg/mL in anisomycin treated extract samples.

sELISA

Supplier Data

Sandwich ELISA - Human ATF2 (phospho T71) ELISA Kit (AB316917)

Example of human ATF2 (phospho T71) standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell Lysate

交差種

Human

アッセイタイプ

Sandwich (quantitative)

検出感度

= 4.573 pg/mL

検出範囲

39.063 - 2500 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human ATF2 (phospho T71) SimpleStep ELISA^® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ATF2 (phospho T71) protein in Cell Lysate. Quantitate Human ATF2 (phospho T71) with 4.573 pg/ml sensitivity.

SimpleStep ELISA^® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details. Our SimpleStep ELISA^® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA^® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA^® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.8" } ]

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回収率

[ { "sample": "Cell Lysate", "range": "91 - 98 %", "average": "= 95" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The ATF2 protein also referred to as ATF-2 or activating transcription factor 2 plays a significant role as a transcription factor in cellular processes. It weighs approximately 75 kDa and is expressed in many tissues with higher levels in the brain heart and skeletal muscle. Functionally ATF2 belongs to the leucine zipper family of proteins facilitating its ability to bind DNA and regulate the expression of genes involved in stress responses development and growth.

Biological function summary

ATF2 takes part in the regulation of gene expression in response to various stimuli. It often forms a complex with other proteins such as c-Jun when binding to the DNA. This complex then influences the transcription of genes that respond to cellular stress and DNA damage. By phosphorylating specific serine residues cellular kinases activate ATF2 which then translocates to the nucleus where it exerts its function.

Pathways

ATF2 integrates into the MAPK and JNK signaling cascades which are important for transmitting stress signals from the cell surface to the nucleus. Through these pathways ATF2 interacts with proteins such as JNK and p38 MAPK modulating the transcription of downstream genes that control cell proliferation apoptosis and differentiation. Its role in these pathways positions ATF2 as a critical node where various signaling inputs merge to influence cellular outcomes.

ATF2 has associations with conditions such as cancer and neurological disorders. Aberrant regulation of ATF2 can contribute to oncogenesis by affecting cell cycle control and apoptosis. For example in melanoma altered ATF2 activity is linked to tumor progression and resistance to apoptosis. Additionally in neurological disorders its interaction with proteins like phospho-c-Jun influences neuronal survival and plasticity implicating ATF2 in pathologies related to neurodegeneration and cognitive dysfunction.

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製品プロトコール

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ターゲットの情報

Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro (PubMed : 10821277). In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.

See full target information ATF2 phospho T71

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websiteProtocolBooklet

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Abcam product promise

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Human ATF2 (phospho T71) ELISA Kit