Human Aquaporin 4 ELISA Kit - Extracellular (ab290982)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.9 pg/ml
- Range: 3.906 pg/ml - 250 pg/ml
- Sample type: Cerebral Spinal Fluid, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Aquaporin 4 ELISA Kit - Extracellular
Aquaporin 4 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Serum 8 10.1% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Serum 3 11.5% -
サンプルの種類
Serum, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
1.9 pg/ml -
検出範囲
3.906 pg/ml - 250 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 90 81% - 98% Cerebral Spinal Fluid 114 95% - 125% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human Aquaporin 4 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Aquaporin 4 protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human Aquaporin 4 with 1.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human Aquaporin 4 Capture Antibody 1 x 600µl 10X Human Aquaporin 4 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human Aquaporin 4 Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. -
組織特異性
Brain - muscle >> heart, kidney, lung, and trachea. -
配列類似性
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
ドメイン
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
翻訳後修飾
Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40%. -
細胞内局在
Membrane. - Information by UniProt
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別名
- AQP 4
- AQP-4
- AQP4
see all -
参照データベース
- Entrez Gene: 361 Human
- Omim: 600308 Human
- SwissProt: P55087 Human
- Unigene: 315369 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab290982の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Sandwich ELISA - Human Aquaporin 4 ELISA Kit (ab290982)Example of human Aquaporin 4 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
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Sandwich ELISA - Human Aquaporin 4 ELISA Kit (ab290982)Interpolated concentration of native Aquaporin 4 was measured in duplicate at different sample concentrations. Undiluted samples are 12.5% cerebrospinal fluid. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS -
Human Aquaporin 4 ELISA Kit (ab290982)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Human Aquaporin 4 ELISA Kit (ab290982)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab290982 は論文での使用が確認できていません。