Human Apolipoprotein CIII ELISA Kit (APOC3) (ab154131)
Key features and details
- Sensitivity: 1.1 ng/ml
- Range: 1.953 ng/ml - 500 ng/ml
- Sample type: Cerebral Spinal Fluid, Milk, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Apolipoprotein CIII ELISA Kit (APOC3)
Apolipoprotein CIII キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 5.4% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 10.4% -
サンプルの種類
Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
1.1 ng/ml -
検出範囲
1.953 ng/ml - 500 ng/ml -
添加回収試験
99 %
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全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Human Apolipoprotein CIII (APOC3) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab154131) is designed for the quantitative measurement of APOC3 in plasma, serum, urine, milk, CSF, and cell culture samples.
An Apolipoprotein CIII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein CIII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein CIII captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Apolipoprotein CIII Antibody 1 x 120µl Apolipoprotein CIII Microplate (12 x 8 well strips) 1 unit Apolipoprotein CIII Standard 2 vials Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. -
組織特異性
Constitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine. -
関連疾患
Defects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL). -
配列類似性
Belongs to the apolipoprotein C3 family. -
翻訳後修飾
O-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan. -
細胞内局在
Secreted. - Information by UniProt
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別名
- APOC3
- APO C3
- Apo CIII
see all -
参照データベース
- Entrez Gene: 345 Human
- Omim: 107720 Human
- SwissProt: P02656 Human
- Unigene: 73849 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154131の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Typical Standard Curve
Representative Standard Curve using ab154131.
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Sandwich ELISA - Apolipoprotein CIII (APOC3) Human ELISA Kit (ab154131)APOCIII measured in biological fluids, background signal subtracted (duplicates +/- SD). -
Sandwich ELISA - Apolipoprotein CIII (APOC3) Human ELISA Kit (ab154131)Standard curve of Hu APOCIII, with background signal subtracted (duplicates; +/- SD).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab154131 は 14 報の論文で使用されています。
- Hsu CC et al. Apolipoprotein C3 induces inflammasome activation only in its delipidated form. Nat Immunol 24:408-411 (2023). PubMed: 36781985
- Kovrov O et al. Lipoprotein size is a main determinant for the rate of hydrolysis by exogenous LPL in human plasma. J Lipid Res 63:100144 (2022). PubMed: 34710432
- Liu P et al. Proteomic Profiling of Cryoglobulinemia. Front Immunol 13:855513 (2022). PubMed: 35677050
- Leiherer A et al. Lipid profiles of patients with manifest coronary versus peripheral atherosclerosis - Is there a difference? J Intern Med 290:1249-1263 (2021). PubMed: 34337800
- Zhang T et al. HDL-associated apoCIII plays an independent role in predicting postprandial hypertriglyceridemia. Clin Biochem 79:14-22 (2020). PubMed: 32045574