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Human Apolipoprotein CI ELISA Kit (Apo C1) (ab108808)

Submit a review Q&A (3)References (5)
Typical Standard Curve

    Key features and details

    • Sensitivity: 36 ng/ml
    • Range: 0.063 µg/ml - 4 µg/ml
    • Sample type: Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    製品の概要

    • 製品名

      Human Apolipoprotein CI ELISA Kit (Apo C1)
      Apolipoprotein CI/Apo-CI キット 製品一覧

    • 検出方法

      Colorimetric

    • 再現性

      Intra-Assay（同時再現性）
      サンプル N 平均値 SD CV%
      Overall 5.5%
      Inter-Assay（日差再現性）
      サンプル N 平均値 SD CV%
      Overall 10.9%

    • サンプルの種類

      Serum, Plasma

    • アッセイタイプ

      Sandwich (quantitative)

    • 検出感度

      = 36 ng/ml

    • 検出範囲

      0.063 µg/ml - 4 µg/ml

    • 添加回収試験

      98 %

    • 全工程の試験時間

      5h 00m

    • ステップ

      Multiple steps standard assay

    • 種交差性

      交差種: Human

    • 製品の概要

      Abcam’s Apolipoprotein CI (APOCI) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein CI concentrations in plasma, serum, cell culture supernatants, cell lysates and tissue samples.


      An Apolipoprotein CI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein CI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein CI captured in plate.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

    • アプリケーション

      適用あり: Sandwich ELISAmore details

    • 試験プラットフォーム

      Microplate

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab108808の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Sandwich ELISA
    Use at an assay dependent concentration.
    特記事項
    Sandwich ELISA
    Use at an assay dependent concentration.

    画像

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108808

    参考文献 (5)

    ab108808 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab108808 は 5 報の論文で使用されています。

    • Yu K  et al. APOC1 as a novel diagnostic biomarker for DN based on machine learning algorithms and experiment. Front Endocrinol (Lausanne) 14:1102634 (2023). PubMed: 36891052
    • Yu K  et al. APOC1 exacerbates renal fibrosis through the activation of the NF-κB signaling pathway in IgAN. Front Pharmacol 14:1181435 (2023). PubMed: 37305534
    • Kovrov O  et al. Lipoprotein size is a main determinant for the rate of hydrolysis by exogenous LPL in human plasma. J Lipid Res 63:100144 (2022). PubMed: 34710432
    • Jin J  et al. iTRAQ-based comparative proteomics analysis reveals specific urinary biomarkers for various kidney diseases. Biomark Med 14:839-854 (2020). PubMed: 32856461
    • Yang J  et al. Peptidome Analysis Reveals Novel Serum Biomarkers for Children with Autism Spectrum Disorder in China. Proteomics Clin Appl 12:e1700164 (2018). PubMed: 29754444

    レビューと Q&A

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    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Question

    I respect what you say but for publication all the journals will not accept any new kit unless it is validate. Could you kind enough to send us one kit of any one of selected proteins so we can validate it.

    Best regards

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    Answer

    Unfortunately I will not be able to send you a sample kit for testing purpose because this is against the company guidelines. All our product are vigorously tested in compatible samples and are fully guaranteed so if journal has any problem we can provide certificate of compliance which explain the kit will do what the datasheet say.

    Once again I am sorry we will not be able to provide you free samples for testing. I can however provide Abtrial offer code if the kits are going to be used in samples or species not listed on the datasheet. By participating in our 100% Abtrial discount programme - a free product can be claimed in exchange of review for this antibody. Please contact me for code if you accept our offer. Please note that purchase for the first kit is necessary.

    More details about the offer can be found at the following link;

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

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    Question

    Hi, just wanted to know how it is going with my request.

    Read More
    Answer

    I am sorry for the delay in getting back to you.

    I can confirm that the antibodies used in this ELISA are polyclonal antibodies raised against the full length Apo CI protein.Theexact epitopes of binding are therefore not known and it is likely that there are multi-binding sites.

    I am sorry that I could not be of more help in this instance, if you have any further questions please do not hesitate to contact us again.

    Read More

    Question

    Dilution of standard?

    Read More
    Answer

    Thank you for your call.

    I can now confirm that in the kit with the lot number1239351 (amount is lotspecific)each vial contains 2 ug of Apo C-I standard.

    We provide 2 vials of Apo C-I standard so the customer can use a fresh vial of standard when running another assay after some time.

    Reconstitute the 2 ug of Apo C-I Standard with 0.5 ml of Diluent to generate a solution of 4 ug/ml. Allow the standard to sit on ice for 10 minutes with gentle agitation prior to making dilutions. Prepare duplicate or triplicate standard points by serially diluting the standard solution (4 ug /ml) 1:2 with Diluent to produce 2, 1, 0.5, 0.25, 0.125 and 0.063 ug/ml solutions. Diluent serves as the zero standard (0 ug/ml). Any remaining solution should be frozen at -200C.

    I hope this information is helpful. Please do not hesitate to contact me with any further questions.

    Read More

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    For licensing inquiries, please contact partnerships@abcam.com